Growth Medium Compound accumulating Minimal Minimal Minimal + Minimal Minimal Mutant medium cysteine cystathionine homocysteine methionine strain Control prototroph in mutant None + + + Met Homocysteine Cystathionine Cysteine Homoserine + Met 2 + Met 3 + Met 4 + + + Homocysteine Methionine CysteineCystathionine Homoserine

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Nitrogen metabolism

Nitrogen metabolism is the synthesis, utilization or uptake, breakdown, and excretion of nitrogenous compounds in the body. The nitrogen metabolism encompasses nitrogen fixation, biosynthesis, and the breakdown of amino acids, purines, and pyrimidine. Since nitrogen-containing compounds are required for various life processes, nitrogen is an important element of life.