mTOR is a cytoplasmic kinase that regulates cell division. Its misregulation can lead to cancer. The canonical mTOR inhibitor, rapamycin, is FDA approved as a cancer treatment. Rapamycin is an allosteric inhibitor that does not bind anywhere near the substrate binding site of the enzyme. A) What is the name of this type of inhibition? B) Sketch a REPRESENTATIVE double-reciprocal/Lineweaver-Burke plot that includes the enzyme kinetics for BOTH the uninhibited and inhibited reaction. Be sure to: 1) Make ABSOLUTELY clear which curve is your uninhibited reaction and which is your inhibited reaction. 2) Label the X and Y axes. 3) Indicate what the X- and Y-intercepts represent with regards to Michaelis-Menten kinetics. C) Can increasing the substrate concentration overcome this type of inhibition? Explain in a sentence or two.
mTOR is a cytoplasmic kinase that regulates cell division. Its misregulation can lead to cancer. The canonical mTOR inhibitor, rapamycin, is FDA approved as a cancer treatment. Rapamycin is an allosteric inhibitor that does not bind anywhere near the substrate binding site of the enzyme.
A) What is the name of this type of inhibition?
B) Sketch a REPRESENTATIVE double-reciprocal/Lineweaver-Burke plot that includes the enzyme kinetics for BOTH the uninhibited and inhibited reaction. Be sure to:
1) Make ABSOLUTELY clear which curve is your uninhibited reaction and which is your inhibited reaction.
2) Label the X and Y axes.
3) Indicate what the X- and Y-intercepts represent with regards to Michaelis-Menten kinetics.
C) Can increasing the substrate concentration overcome this type of inhibition? Explain in a sentence or two.
Given;
mTOR: Cytoplasmic kinase- regulates cell division
Rapamycin: Allosteric inhibitor
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