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3) Histone deacetylases (HDACs) are zinc-dependent enzymes that catalyse the conversion of acetyllysine to lysine. HN HDAC NH₂ a) Propose the mechanism for the HDAC-catalysed reaction. b) What is the role of zinc ion in HDAC catalysis? c) Draw the pH-activity profile for HDAC-catalysed conversion of acetyllysine to lysine. Explain. d) Suggest two acetyllysine analogues that are substrates for HDACS. One should be a better substrate than acetyllysine, whereas the other one should be a worse substrate than acetyllysine. Explain. Mutations in the HDAC sequence could lead to a loss of the catalytic function. Which mutations are problematic? e) f) Lysine acetylation is a reversible reaction using acetyl-CoA as a co-substrate. Which coenzymes are required for synthesis of acetyl-CoA in nature? Propose the mechanism.

3) Histone deacetylases (HDACs) are zinc-dependent enzymes that catalyse the conversion of acetyllysine to lysine. HN HDAC NH₂ a) Propose the mechanism for the HDAC-catalysed reaction. b) What is the role of zinc ion in HDAC catalysis? c) Draw the pH-activity profile for HDAC-catalysed conversion of acetyllysine to lysine. Explain. d) Suggest two acetyllysine analogues that are substrates for HDACS. One should be a better substrate than acetyllysine, whereas the other one should be a worse substrate than acetyllysine. Explain. Mutations in the HDAC sequence could lead to a loss of the catalytic function. Which mutations are problematic? e) f) Lysine acetylation is a reversible reaction using acetyl-CoA as a co-substrate. Which coenzymes are required for synthesis of acetyl-CoA in nature? Propose the mechanism.

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
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3) Histone deacetylases (HDACs) are zinc-dependent enzymes that catalyse the conversion of acetyllysine to lysine. e) HN a) Propose the mechanism for the HDAC-catalysed reaction. b) What is the role of zinc ion in HDAC catalysis? c) d) f) HDAC NH₂ Draw the pH-activity profile for HDAC-catalysed conversion of acetyllysine to lysine. Explain. Suggest two acetyllysine analogues that are substrates for HDACs. One should be a better substrate than acetyllysine, whereas the other one should be a worse substrate than acetyllysine. Explain. Mutations in the HDAC sequence could lead to a loss of the catalytic function. Which mutations are problematic? Lysine acetylation is a reversible reaction using acetyl-CoA as a co-substrate. Which coenzymes are required for synthesis of acetyl-CoA in nature? Propose the mechanism.
Transcribed Image Text:3) Histone deacetylases (HDACs) are zinc-dependent enzymes that catalyse the conversion of acetyllysine to lysine. e) HN a) Propose the mechanism for the HDAC-catalysed reaction. b) What is the role of zinc ion in HDAC catalysis? c) d) f) HDAC NH₂ Draw the pH-activity profile for HDAC-catalysed conversion of acetyllysine to lysine. Explain. Suggest two acetyllysine analogues that are substrates for HDACs. One should be a better substrate than acetyllysine, whereas the other one should be a worse substrate than acetyllysine. Explain. Mutations in the HDAC sequence could lead to a loss of the catalytic function. Which mutations are problematic? Lysine acetylation is a reversible reaction using acetyl-CoA as a co-substrate. Which coenzymes are required for synthesis of acetyl-CoA in nature? Propose the mechanism.
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