A. Which one of the following is an example of affinity chromatography? Select all that apply.a) Hormone – receptor interactionb) Enzyme – substrate interactionc) Antigen-antibody interactiond) All of the abovee) Cytochrome C – DEAE matrixB. If your sepharose beads are covalently attached to adrenaline hormone, which of the followingprotein is most likely purified by affinity chromatography?a) Receptor tyrosine kinase (RTK)b) Calmodulinc) GPCRd) Protein kinase AC. Which of the following malate dehydrogenase (MDH) has a 'peptide epitope tag'?a) MDH-HAb) MDH-GSTc) MDH-GFPe) All of themD. To elute target proteins from an affinity chromatography matrix, which of the followingconditions would be the most appropriate?a) Low salt concentrationsb) High salt concentrationsc) Adding a soluble ligand that competes with the affinity tagged protein for binding to the columnd) Just keep washing buffer through the column, isocratic elution

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Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

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Problem 1P

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A. Which one of the following is an example of affinity chromatography? Select all that apply. a) Hormone – receptor interaction d) All of the above b) Enzyme - substrate interaction c) Antigen-antibody interaction e) Cytochrome C – DEAE matrix