Which technique is preferred in the separation of fatty acyl methyl esters from a mixture?a) Gas-liquid chromatographyb) Absorption chromatographyc) TLCd) Centrifugation

Human Anatomy & Physiology (11th Edition)
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Which technique is preferred in the separation of fatty acyl methyl esters from a mixture?
a) Gas-liquid chromatography
b) Absorption chromatography
c) TLC
d) Centrifugation

Expert Solution
Step 1
  • Chromatography is defined as the physical method that can be used for separating different compounds. 
  • The principle of chromatography involves the sample that is supposed to be analyzed is allowed to interact with two different phases i.e. a mobile phase and a stationary phase.
  • Chromatography can be of different types based on the type of immiscible phase used for the separation.
  • The centrifugation technique is also a separation technique that principally works on the phenomena of centrifugal force. 
  • The objects that have higher mass feel the greater force and get sedimented at the bottom while the lighter particles remain above to that. 
Step 2
  • In gas-liquid chromatography, the stationary phase is liquid while the mobile phase is a gas. The gas-liquid chromatography is effectively used for the separation.
  • Chromatography is based on the phenomena of adsorption and there is no chromatographic technique present that is called absorption chromatography.
  • The thin-layer chromatography (TLC) is helpful in the detection of a compound from the given solvent.  
  • The centrifugation technique is only used in the separation of solute from the solvent when there is a considerable mass difference.
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