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A:
Q: What is recombinant DNA? How can it be used to produce human proteins in bacteria?
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Q: Is it possible to obtain DNA in cloverleaf configuration? Explain
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Q: t is the principle of gel electrophoresis?
A: The purity of isolated nucleic acids is determined using electrophoresis.
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A: Electrophoresis It is a biochemical technique to separate charged molecules under the influence of…
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Q: What is gene cloning? What is bacterial transformation? What is the difference between the two…
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Q: What is used to identify the size of a DNA molecule during gel electrophoresis?
A:
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Q: Why is there a need for replica plating? How is it performed?
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Q: What causes the separation of DNA fragments during agarose gel electrophoresis?
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Q: What are plasmids? What is the importance of plasmids for the recombinant DNA technology?
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Which of the chemical compounds below can be used to label DNA for flow cytometry?
A )Nocadazole
B) Propidium Iodide (PI)
C) Triton-X (Tx)
D) Bromodeoxyuridine (BrdU)
E) Phosphate Buffered Saline (PBS)
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- 7) One of the following antibiotics doesn’t work through the same class of molecules as the rest: a) Levofloxacin b) Rifampin c) Moxifloxacin d) Daptomycin e) Ciprofloxacin 8) In the formula [d=0.5 / n sin , d stands for: a) distance between 2 points b) distance from the slide to the objective c) Focal distance of the objective lens d) Focal distance of the ocular lens e) none of the above9) In the formula [d=0.5 / n sin , stands for: a) angle of reflection b) angle of refraction c) angle of incidence d) angle of inclination e) angle of perception10) In the formula [d=0.5 / n sin , stands for: a) angle of incidence b) speed of the illuminative source/light c) Nature of illuminative source/light d) wavelength illuminative source/light e) temperature of illuminative source/light20) which of the following types of information is provided by a suffix? A) numbers b) positions c) times d) diagnostic proceduresWhich of the following is true of the gel electrophoresis shown? A) the power source has not been turned on yet. B) the three wells contained the same DNA molecules. C) well #1 had fewer molecules of DNA than well #2 or #3 D) well #3 had more different sized molecules of DNA than well #1 or #2 Which of the following statements is TRUE about the band of molecules labeled X ( close to where it says positive electrode). A) it contains DNA fragments that are shorter than the ones in any of the other bands. B) it contains fewer DNA molecules than any of the other bands from lane 3. C) it contains more negatively charged particles than any other band on the gel. D) it contains fewer genes than any of the other bands on the gel.
- Which of the following statements is correct about column chromatography? a) Resolution improves as column length increases b) Mobile phase is a porous solid material kept in the column with appropriate chemical characteristics c) Stationary phase is a buffered solution that percolates through the mobile phase d) Large proteins exit the column faster than tiny proteinsRegarding the cultivation of animal cells in vitro, it is correct to state that: *(Only one statement is correct) a) Transformed cells differ from normal and established cells in that they have the ability to proliferate indefinitely in culture b) Transformed cells derive from isolated and dissected tumors, constituting cell lines that have limited cultivation time c) The genetic instability presented in normal cells makes them preferential models for the production of recombinant proteins d) Cells cultured in adhesion must be periodically expanded, through the use of enzymes such as trypsin e) The metabolism of carbon sources in the culture medium by animal cells generates products such as lactate, which positively influence their productivity-Two hundred patients without chest pain were screened with a new biochemical test that showed 190 had a negative screening result. What is the test's specificity? a) 80% b) 95% c) 90% d) 75%
- What method do ELISA’s utilize in their measurement? a) light absorption (eg Abs480nm) b) b+–radiation c) combination of absorption and radiation d) electrophoresisQ1/ Choose the Right Answer 1. Biomaterials science is not significant monetarily, but the magnitude of the field is extremely important since biomaterials are used to save millions of lives. a) Ttrue , b) False 2. Platinum can be used in pacemaker because of a) low Wight, b) high threshold potential , c) high corrosion resistance ,d) none of above 3. Which one of the following is not correct? a) Biocompatibility is the ability of a material to perform within an appropriate host response. b) Bioactivity is the characteristic that not allows the material to form a bond with living tissue c) Biodegradability is the breakdown of the implant due to chemical or cellular interaction. d) all of above 4. Which of the following phases of dental amalgam has minimum strength? a) Gamma 1, b) Gamma 2, c) Gamma 3, d) Both a and b 5. A biomaterial has to be biocompatible, which means several things. Which one of these is wrong? a) The body must not fight or attack the biomaterial b)The biomaterial…Q1/ Choose the Right Answer 1. Biomaterials science is not significant monetarily, but the magnitude of the field is extremely important since biomaterials are used to save millions of lives. a) Ttrue , b) False 2. Platinum can be used in pacemaker because of a) low Wight, b) high threshold potential , c) high corrosion resistance ,d) none of above 3. Which one of the following is not correct? a) Biocompatibility is the ability of a material to perform within an appropriate host response. b) Bioactivity is the characteristic that not allows the material to form a bond with living tissue c) Biodegradability is the breakdown of the implant due to chemical or cellular interaction. d) all of above
- Regarding the use of animal cells in culture, it is correct to state that: * (a) Low cost culture media composed of mineral salts and amino acids are used (b) Cells must be kept in semi-solid media, allowing them to come into contact with the atmosphere rich in carbon dioxide. (c) Greenhouses with controlled temperature and atmosphere composed predominantly of carbon monoxide are used (d) On an industrial scale, culture media supplemented with serum of animal origin are used, which makes them media of indefinite composition (e) Culture media must not be sterilized by autoclaving, preferably filtration and/or ionizing radiation methods are used11 Enzyme assay A) Always involves asking a substrate B) Can be very smoke or very complex C) Always involves ultraviolet light D) A-C are incorrect 12 Isolation of chromosomal (total) DNA using a spin column A) Involves a filtration step to remove many different molecules B) Lysis is an optional step C) Elution is not necessary D) Results in a very pure protein preparation 13 Isolation of chromosomal (total) DNA using a spin column A) Cells must be used using an appropriate method B) Cell lysis is required C) Elution of DNA required regardless of the cell type used in the experiment D) A-C are correct. 14 Isolation of chromosomal (total) DNA using a spin column A) Washing the spin column is optional B) DNA must be eluted from the spin column C) Does not involve centrifugation D) Only molecules that are supposed to bind to the column do so.11. ● Possible methods for the quantitative determination of sodium bicarbonate for injection are: A) Acidimetry B) Alkalimetry C) Complexometry D) Iodometry E) Refractometry ● Calcium chloride is available in dosage forms: A) Powder for preparation of injections B) Solution for injection 10% in ampoules C) Tablets of 0.3 g D) Solution for injection 25% in ampoules E) Dry dosed powder