Answered: Currently I am working with qPCR…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Currently I am working with qPCR quantification specific for Lactobacillus bacteria. I found in some cases, the sample showing multiple peaks of melting curves while others looks perfect, so do the amplification plot duplication. Any one have an opinion about this issues? Does it caused by sample DNA quality, the omount of the bacteria detected, or primers problem? I am condisering that this condition reflecting the low abundance of the specific bacteria inside its sample.

Amplification Plot
1000
100
10 10.0
0.1
0.01
0.001
0.0001
0.00001
0.000001
2
4
10
12
14
16
18
20
22
24
26
28
30
32
34
36
38
40
Cycle
Legend
A
D
E
G.
ARn

Melt Curve
760000.0
660000.0
580000.0
460000.0
360000.0
260000.0
160000.0
60000.0
-40000.0
85.0
70.0
75.0
80.0
85.0
90.0
95.0
Tm: 82.91
Temperature ("C)
Legend
В
D
E IF
Derivative Reporter (-R)

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