What is not true for Sequence tagged site (STS) markers:cannot be mapped by fluorescence in situ hybridization (FISH)subset of STS markers are known as expressed sequence tag (EST) markersOcan readily be screened by a PCR assayO short DNA sequences that occur at a unique location in the genome

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Answered: What is not true for Sequence tagged…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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A number of advances have been made in biotechnology. CRISPR/Cas9 one of the most controversial, and has had a lot of media attention in recent years. It is a method by which scientists can precisely edit DNA sequences at exact locations. Benefits obviously include the potential to “repair” mutated genes that cause disease. In fact, preliminary results from one of the earliest clinical trials of CRISPR/Cas9 provide evidence that the technique is safe and feasible to use for treating human diseases. What other potential applications of this application do you see (you can use any organisms to illustrate your answer)? What are the potential dangers or downsides of using this technology? Do you think this technology should be used in gene editing in humans? Explain your stance.
The following statements are true about common gene cloning procedures except: -DNA plasmids can help move around genes into other cells - Restriction enzymes are very important for cutting up and linking chunks of DNA -We make it so that genes from plants or animals are expressed in bacteria so the products can be harvested - DNA plasmids are chunks of chromosomal DNA used for cloning
In a PCR assay, what's the purpose of each temperature step at: -heating the sample to 50 C -heating the sample to 72 C -heating the sample to 95 C options to match: - allows primers to anneal to the template DNA - promotes synthesis of new DNA - separates the DNA strands in the samples
● Gene lacZ fliE Please fill in the table below for the desired mutations from section five. Please reword any pathway information you find and reference the source (not just ecocyc.com) omp A lexA Size of gene (bp) 3075 315 rpoA nagC 1221 990 1041 609 Protein Pathway Expected size of PCR (bp) name
If a researcher attempted to do a PCR amplification of the slc24a5 gene in the gol1 mutatant line, would their attempts have been successful? Why or why not? No; PCR amplification will not be successful because the gene is deleted in the golb1 mutatant line. No; PCR amplification will not be successful because there is a point mutation in the coding region of the gene. Yes; this mutant line had a point mutation that would not have impacted PCR. Yes; although the gene switch was mutated, it did not impact the promotor sequence. O O O O
CRISPR One of the more recent advances in biotechnology is the development of the CRISPR gene modification tool. Match the following descriptions the the key players in this technology. NOTE: If you want to change your selection, you'll need to delete the one you already chose. After you delete it, the list of choices will pop back up and you can make a different choice. CRISPR technology Specialized stretches of DNA with nucleotide repeats and Cas9 protein spacers Enzyme that cuts DNA crRNA Guides the enzyme to the target site CRISPR DNA Adapted from the natural defense mechanism of bacteria and archaea
In the following "gene library" cloning experiment Digested genomic DNA AmpR gene TCR gene TCR is tetracycline resistant marker, AmpR is ampicillin resistant marker and BamHI is the unique restriction enzyme on plasmid. A PhD student digests/cuts the plasmids with BamHI restriction enzyme and the genomic DNA with EcoRI restriction enzyme. After performing the cloning experiment and obtaining colonies on a selection plate, the obtained cells will be ..... (Hint: this question is even more challenging; the PhD student was later demoted to an MSc student). a) resistant to ampicillin and tetracycline b) sensitive to tetracycline and ampicillin c) resistant to tetracycline and sensitive to ampicillin d) resistant to ampicillin and sensitive to tetracycline e) sensitive to ampicillin and tetracycline BamHI
Which of the following is correct regarding sequencing by synthesis? All four nucleotides are added simultaneously during the sequencing phase. All four nucleotides have fluorescent tags. Homopolymer error rates are significantly lower than other methods of second-generation sequencing. All of the above
If the researchers carry out a PCR for this gene from the CD8+ T cells, what result would you expect?Explain.
An organism no longer needs to express a particular gene. What is one strategy it might use of the methods discussed in class? Select all that apply. 0.000 de-methylate C bases in the genome acetylate C bases in the genome methylate C bases in the genome acetylate histones de-acetylate histones de-acetylate C bases in the genome

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What is not true for Sequence tagged site (STS) markers: cannot be mapped by fluorescence in situ hybridization (FISH) subset of STS markers are known as expressed sequence tag (EST) markers O can readily be screened by a PCR assay short DNA sequences that occur at a unique location in the genome