Which of the following is a unique attribute of CRISPR/Cas9 compared to restriction enzymes Group of answer choices it can be targeted to any specific region of the genome as long as there is an adjacent PAM motif it was first discovered in bacterial cells it can cut double-stranded DNA sequences it can be used for molecular cloning experiments

Q: NOT a DNA-based method

A: This is a method which refers to the technique or approach that utilizes the DNA molecules or…

Q: During pcr primers can:  become graded by rnases be covslently linked to the template dtrand fall…

A: PCR stands for polymerase chain reaction. It is used to amplify the specific region of gene.

Q: Why must the gene be inserted into a vector for it to be cloned?

A: Vectors are carriers of target DNA which are readily used in recombinant DNA technology. The most…

Q: Which of the following would be the best assay to use to determine if a protein binds to RNA? O…

A: Gel electrophoresis is a laboratory technique used to separate and analyze charged molecules, such…

Q: CRISPR-Cas9

A: Both statements are true.

Q: Which of the following is NOT true about transduction? O The bacteriophage's own viral DNA is the…

A: Bacteriophage transduction is a type of genetic transfer in which bacterial DNA is transmitted from…

Q: How is bacterial DNA safe from digestion by Restriction Endonucleases?     The plasmid of the…

A: Question-  How is bacterial DNA safe from digestion by Restriction Endonucleases?   A) The…

Q: CRISPR-CAS9 is a: s a: alien weapon to control our minds a eukaryotic gene protein matrix type of…

A: CRISPR-Cas9, which is short for clustered regularly interspaced short palindromic repeats and…

Q: Draw an Illustration of the steps in restriction digestion and PCR [ Please make it clean and…

A: Restriction digestion is the process of cutting the DNA sequence at a particular nucleotide sequence…

Q: Which of the following types of mutations would be produced by repair of a CRISPR-targeted double…

A: Non Homologous End joining is described as the pathway required for the repairing of those double…

Q: PCR is used for cloning DNA and after about 30 cycles, the number of copies DNA will have increased…

A: The enzyme DNA polymerase which is used for PCR reactions is called Taq DNA polymerase which is…

Q: Which molecule is responsible for producing the double-stranded breaks that initiate CRISPR genome…

A: The biochemical molecule that is built up with two polynucleotide chains is called Deoxyribonucleic…

Q: Which of the following will MOST LIKELY yield a construct containing an insert in the correct…

A: A vector construct, also known as a cloning vector or expression vector, is a DNA molecule that is…

Q: Why is it important to sequence positive clones derived from PCR cloning? Group of answer choices

A: PCR is a technique of amplification of a given molecule of DNA to increase its copy number.

Q: WHICH OF THE FOLLOWING TECHNIQUE IS NOT USED TO MANIPULATE THE GENOME 1. siRNA 2. ZF (zinc finger)…

A: The entirety of an organism's genetic code, or DNA, is known as its genome. It includes all the…

Q: however it is still unclear that the function of th gene is and the lab director asks you for…

A: Previously getting the idea of the genome of any organism was very difficult but, Recombinant DNA…

Q: Which of the following is a correct statement about CRISPR-Cas-9 gene editing? Group of answer…

A: CRISPR-Cas9 is a widely used genome editing technology as it enables scientists to alter genes in…

Q: WHAT is the subject of the figure above? WHAT is the main message of the figure? Give a BRIEF but…

A: Biotechnology is the use of our comprehension of biological processes to develop practical clinical…

Q: is used to seal the phosphodiester backbones between vector and insert. O DNA polymerase O Ligase…

A: Phosphodiester bonds are formed when exactly two of the hydroxyl groups in the phosphoric acid react…

Q: What if you designed a CRISPR/cas9 assay for a gene knockout, but there's no PAM sequence…

A: CRISPR is a group of DNA sequences present in the genomes of bacteria and archaea, which are…

Q: Which of these statements is INCORRECT about restriction enzymes

A: A restriction enzyme is a protein isolated from bacteria. It help to  cleaves DNA sequences at…

Q: What is not true for Sequence tagged site (STS) markers: cannot be mapped by fluorescence in situ…

A:

Q: Which of the following is a correct statement about the primers used in the ALU insertion PCR…

A: Answer -  Option B - The primers for both + and - alleles are the same and are on flanking regions…

Q: Which of the following is TRUE of next generation sequencing technology? It is required for CHIP-seq…

A: Next generation sequencing (NGS) is a massively used sequencing technology that has revolutionized…

Q: Which of the following is not a component of Sanger sequencing? OddNTPs with fluorescent tags…

A: Question 1 explantion: The component not typically used in Sanger sequencing is restriction enzymes.…

Q: Differentiate between TWO of the following pairs: Genetic and a restriction map Southern and…

A: The mapping of DNA refers to the identification of the location of particular genes. Genetic map and…

Q: Which of the following best explains the natural function to the tumor-inducing (Ti) plasmid? It…

A: The deoxyribonucleic acid (DNA) is the double-stranded molecule that is the genetic material in most…

Q: One improvement of CRISPR-Cas9 technology over the classical recombinant DNA technology is its…

A: CRISPR/Cas9 is very fascinating technology discovered till date. Its a gene editing tool widely used…

Q: What is the role of the following molecules in the CRISPR mechanism? *

A: CRISPR stands for clustered regularly interspaced short palindromic repeats. These are a set of DNA…

Q: Which of the following protocols is BEST used for introducing exogenous DNA into a cell with a thick…

A: The gene gun approach is the most effective method for transferring exogenous DNA into a cell with a…

Q: During RT-PCR, a poly-T primer and reverse transcriptase is used to turn all the mRNA to DNA. Why is…

A: RT-PCR, or reverse transcription polymerase chain reaction, is a widely used technique for the…

Q: Which of the following mutagens can substitute bases in DNA because of structural similarity?…

A: A mutagen is defined as any physical or chemical substance that can cause a mutation by altering an…

Q: The following DNA sequence has been identified and named ‘Gene Z’. It is thought that this sequence…

A: PCR is a polymerase chain reaction, this is a biotechnological process which is used to amplify the…

Q: The genomic DNA of a bacterial cell is not destroyed by the cell’s own restriction enzymes because…

A: BASIC INFORMATION  RESTRICTION ENZYME  they are also known as molecular scissors it was in the year…

Q: Some mutations can be detected using restriction enzymes. These mutations can create a restriction…

A: Introduction :-Microsatellites, also known as short tandem repeats (STRs), are short sequences of…

Q: Where did the Crispr system first originate and what is its purpose in nature? What is the advantage…

A: We are answering first 4 questions. For rest of questions pls repost.

Q: Which of the following components are required for the polymerase chain reaction (PCR)? Select all…

A: The components that are required for PCR are-  dNTPs, DNA polymerase, Oligonucleotide primers

Q: CRISPR-Cas9 can be used by scientists to edit genes. This system was not created in a lab, however,…

A: CRISPR-Cas 9 (Clustered Regularly Interspaced Short Palindromic Repeats) is a natural mechanism of a…

Q: Genetically modified Atlantic salmon have recently been approved for human consumption. These…

A: CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) refers to a specific DNA sequence…

Q: A The impact of temperature on gene expression enzymatic differences at different temperatures The…

A: RNA-Seq is a sequencing technique that employs next-generation sequencing to reveal the presence and…

Q: Sequencing analysis Use the table below to record your sequence analysis results. Batch Gene Colony…

A: Mutagenesis is a process that modifies an organism's genetic makeup and causes a change in its DNA…

Q: What is the function of gRNA in CRISPR-Cas technologies?It cuts DNA at a targeted sequence and…

A: Introduction There are two types of nucleic acids present in our cell, DNA and RNA. DNA acts as a…

Q: In a PCR assay, what's the purpose of each temperature step at: -heating the sample to 50 C…

A: PCR (polymerase chain reaction) is a technique for analysing a short sequence of DNA (or RNA) also…

Q: Although CRISPR uses some of the same viral vectors as "gene therapy," which was pioneered in the…

A: Gene therapy is the process of changing the genes in the body's cells in order to treat or prevent…

Q: In your experiment there will be 4 different experimental samples labeled A-D (please see Table 3 on…

A: The objective of the question is to understand the implications of observing no bacterial colonies…

Q: DNA RNA Protein Gel electrophoresis Transfer to paper DNA* CDNA* Antibody* Add probe Image specific…

A: The image above shows DNA / RNA / protein  are transferred to nitrocellulose membrane paper known as…