Which of the following is a unique attribute of CRISPR/Cas9 compared to restriction enzymes Group of answer choices it can be targeted to any specific region of the genome as long as there is an adjacent PAM motif it was first discovered in bacterial cells it can cut double-stranded DNA sequences it can be used for molecular cloning experiments

Q: is possible to buy a CRISPR kit that you can do in your own home. Is this a good idea? After…

A: In 2013, the Zhang lab successfully published the first method to engineer CRISPR to edit the genome…

Q: NOT a DNA-based method

A: This is a method which refers to the technique or approach that utilizes the DNA molecules or…

Q: During pcr primers can:  become graded by rnases be covslently linked to the template dtrand fall…

A: PCR stands for polymerase chain reaction. It is used to amplify the specific region of gene.

Q: Which of the following would be the best assay to use to determine if a protein binds to RNA? O…

A: Gel electrophoresis is a laboratory technique used to separate and analyze charged molecules, such…

Q: CRISPR-Cas9

A: Both statements are true.

Q: Direct modification of genes may be achieved using what? (Choose one below): DNA microarray…

A: DNA microarray is a collection of  DNA spots attached to a solid surface. It is used to measure the…

Q: Which of the following is NOT true about transduction? O The bacteriophage's own viral DNA is the…

A: Bacteriophage transduction is a type of genetic transfer in which bacterial DNA is transmitted from…

Q: CRISPR-CAS9 is a: s a: alien weapon to control our minds a eukaryotic gene protein matrix type of…

A: CRISPR-Cas9, which is short for clustered regularly interspaced short palindromic repeats and…

Q: Draw an Illustration of the steps in restriction digestion and PCR [ Please make it clean and…

A: Restriction digestion is the process of cutting the DNA sequence at a particular nucleotide sequence…

Q: Which of the following types of mutations would be produced by repair of a CRISPR-targeted double…

A: Non Homologous End joining is described as the pathway required for the repairing of those double…

Q: PCR is used for cloning DNA and after about 30 cycles, the number of copies DNA will have increased…

A: The enzyme DNA polymerase which is used for PCR reactions is called Taq DNA polymerase which is…

Q: CRISPR techniques allow scientists to modify specific genes while sparing all others, thus…

A: Genetic diseases are the hereditary diseases that are very difficult to treat. With the advancement…

Q: Which of the following will MOST LIKELY yield a construct containing an insert in the correct…

A: A vector construct, also known as a cloning vector or expression vector, is a DNA molecule that is…

Q: In CRISPR genome editing technology, when the Cas9 protein cleaves the target DNA sequence, what…

A: The microorganism depends on multiple defense strategies that support them to resist viral attack.…

Q: WHICH OF THE FOLLOWING TECHNIQUE IS NOT USED TO MANIPULATE THE GENOME 1. siRNA 2. ZF (zinc finger)…

A: The entirety of an organism's genetic code, or DNA, is known as its genome. It includes all the…

Q: Which of the following scenarios could use Next-Gen sequencing to achieve its purpose? Detect…

A: Genomic research has been transformed by next-generation sequencing (NGS), a massively parallel or…

Q: however it is still unclear that the function of th gene is and the lab director asks you for…

A: Previously getting the idea of the genome of any organism was very difficult but, Recombinant DNA…

Q: Select the definition of a microsatellite. short pieces of DNA surrounding the periphery of the…

A: Answer

Q: Which of the following is a correct statement about CRISPR-Cas-9 gene editing? Group of answer…

A: CRISPR-Cas9 is a widely used genome editing technology as it enables scientists to alter genes in…

Q: WHAT is the subject of the figure above? WHAT is the main message of the figure? Give a BRIEF but…

A: Biotechnology is the use of our comprehension of biological processes to develop practical clinical…

Q: is used to seal the phosphodiester backbones between vector and insert. O DNA polymerase O Ligase…

A: Phosphodiester bonds are formed when exactly two of the hydroxyl groups in the phosphoric acid react…

Q: What if you designed a CRISPR/cas9 assay for a gene knockout, but there's no PAM sequence…

A: CRISPR is a group of DNA sequences present in the genomes of bacteria and archaea, which are…

Q: Which of these statements is INCORRECT about restriction enzymes

A: A restriction enzyme is a protein isolated from bacteria. It help to  cleaves DNA sequences at…

Q: Which of the following is a correct statement about the primers used in the ALU insertion PCR…

A: Answer -  Option B - The primers for both + and - alleles are the same and are on flanking regions…

Q: Which of the following is TRUE of next generation sequencing technology? It is required for CHIP-seq…

A: Next generation sequencing (NGS) is a massively used sequencing technology that has revolutionized…

Q: What is the role of a 'vector' in recombinant DNA technology? acts as a carrier for a piece of DNA…

A: The vectors help in carrying and integrating the specified gene. These form a really important a…

Q: Differentiate between TWO of the following pairs: Genetic and a restriction map Southern and…

A: The mapping of DNA refers to the identification of the location of particular genes. Genetic map and…

Q: Which of the following best explains the natural function to the tumor-inducing (Ti) plasmid? It…

A: The deoxyribonucleic acid (DNA) is the double-stranded molecule that is the genetic material in most…

Q: One improvement of CRISPR-Cas9 technology over the classical recombinant DNA technology is its…

A: CRISPR/Cas9 is very fascinating technology discovered till date. Its a gene editing tool widely used…

Q: Please place the stages of a CRISPR-cas9 gene editing workflow in the correct order below. Note,…

A: CRISPR-Cas9 gene editing is a powerful molecular tool that allows scientists to selectively modify…

Q: Why does one need a vector to clone a DNA

A: Cloning is the method of generating the number of copies of desired DNA I  the host.

Q: What is the role of the following molecules in the CRISPR mechanism? *

A: CRISPR stands for clustered regularly interspaced short palindromic repeats. These are a set of DNA…

Q: Which of the following is a type of transposition that involves the movement of DNA within a genome…

A: DNA transposition is the movement of DNA sequence from one location to another. This movement is…

Q: make a mutation to disable the ACE2 gene (this gene encodes the protein that SARS-CoV-2 uses to…

A: We can edit genes with the technology known as CRISPR. In prokaryotic organisms such as bacteria and…

Q: During RT-PCR, a poly-T primer and reverse transcriptase is used to turn all the mRNA to DNA. Why is…

A: RT-PCR, or reverse transcription polymerase chain reaction, is a widely used technique for the…

Q: Which of the following mutagens can substitute bases in DNA because of structural similarity?…

A: A mutagen is defined as any physical or chemical substance that can cause a mutation by altering an…

Q: The genomic DNA of a bacterial cell is not destroyed by the cell’s own restriction enzymes because…

A: BASIC INFORMATION  RESTRICTION ENZYME  they are also known as molecular scissors it was in the year…

Q: Some mutations can be detected using restriction enzymes. These mutations can create a restriction…

A: Introduction :-Microsatellites, also known as short tandem repeats (STRs), are short sequences of…

Q: CRISPR/Cas9 can be used in genome editing. Among the following statements, which one is correct?…

A: Bacteria have evolved various mechanism to resist against the viruses like bacteriophage some of…

Q: RNA sequencing can detect DNA mutations typically easier to analyze can detect transcripts with very…

A: Microarray technology is used to measure the expression levels of thousands of genes simultaneously,…

Q: PCR primers are designed to only replicate the N gene sequence of the viral genome. Part of the N…

A: Polymerase chain reaction, or PCR, is a molecular biology technique to make many copies of a…

Q: R plasmid R determinant CRISPR tracrRNA sgRNA E = A CRISPR nuclease that can cut foreign DNA and…

A: Molecular techniques are used to study the biological activities at a molecular level. These…

Q: A number of advances have been made in biotechnology. CRISPR/Cas9 one of the most controversial, and…

A: CRISPR/Cas9 is found in the prokaryote system Cas 9 is an enzyme that cut DNA

Q: Where did the Crispr system first originate and what is its purpose in nature? What is the advantage…

A: We are answering first 4 questions. For rest of questions pls repost.

Q: Sequencing analysis Use the table below to record your sequence analysis results. Batch Gene Colony…

A: Mutagenesis is a process that modifies an organism's genetic makeup and causes a change in its DNA…

Q: CRISPR techniques allow scientists to modify specific genes while sparing all others, thus…

A: According to the question, we have to explain how CRISPR Technology helps to create better…

Q: What is the function of gRNA in CRISPR-Cas technologies?It cuts DNA at a targeted sequence and…

A: Introduction There are two types of nucleic acids present in our cell, DNA and RNA. DNA acts as a…

Q: Although CRISPR uses some of the same viral vectors as "gene therapy," which was pioneered in the…

A: Gene therapy is the process of changing the genes in the body's cells in order to treat or prevent…

Q: In your experiment there will be 4 different experimental samples labeled A-D (please see Table 3 on…

A: The objective of the question is to understand the implications of observing no bacterial colonies…