R plasmid R determinant CRISPR tracrRNA sgRNA E = A CRISPR nuclease that can cut foreign DNA and destroy it. E Move a new gene into a genome. ||| Small circle of extragenomic DNA that contains antibiotic resistant genes. Antibiotic resistant genes. Trans-acting ('tracer") CRISPR RNA segment as a

Biology (MindTap Course List)
11th Edition
ISBN:9781337392938
Author:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg
Publisher:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg
Chapter15: Dna Technology And Genomics
Section: Chapter Questions
Problem 10TYU: DNA molecular with complementary sticky ends associate by (a) covalent bonds (b) hydrogen bonds (c)...
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sgRNA
Cas9
Knock-in
Knock-out
Knock-down
|||
III
|||
Trans-acting ('tracer) CRISPR RNA segment as a
stem-loop structure that binds to Cas and gRNA.
Interfere with mRNA of a gene so that it cannot be
expressed.
Remove a gene from a genome.
Piece of CRISPR RNA segment that finds invading
foreign DNA.
Clustered regularly interspaced short palindromic
sequences in bacterial that contain repetitive viral
information that can be expressed to interfere with
viral DNA as part of a prokaryotic immune response.
Transcribed Image Text:sgRNA Cas9 Knock-in Knock-out Knock-down ||| III ||| Trans-acting ('tracer) CRISPR RNA segment as a stem-loop structure that binds to Cas and gRNA. Interfere with mRNA of a gene so that it cannot be expressed. Remove a gene from a genome. Piece of CRISPR RNA segment that finds invading foreign DNA. Clustered regularly interspaced short palindromic sequences in bacterial that contain repetitive viral information that can be expressed to interfere with viral DNA as part of a prokaryotic immune response.
R plasmid
R determinant
CRISPR
tracrRNA
sgRNA
M
III
III
III
A CRISPR nuclease that can cut foreign DNA and
destroy it.
Small circle of extragenomic DNA that contains
antibiotic resistant genes.
Move a new gene into a genome.
Antibiotic resistant genes.
Trans-acting ('tracer") CRISPR RNA segment as a
stem-loop structure that binds to Cas and gRNA.
Interfere with mRNA of a gene so that it cannot be
expressed.
Remove a gene from a genome.
Transcribed Image Text:R plasmid R determinant CRISPR tracrRNA sgRNA M III III III A CRISPR nuclease that can cut foreign DNA and destroy it. Small circle of extragenomic DNA that contains antibiotic resistant genes. Move a new gene into a genome. Antibiotic resistant genes. Trans-acting ('tracer") CRISPR RNA segment as a stem-loop structure that binds to Cas and gRNA. Interfere with mRNA of a gene so that it cannot be expressed. Remove a gene from a genome.
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