If the AMP was mistakenly left out of the LB/AMP/X-gal/IPTG agar media E. coli cells transformed with which of the following could grow on the plate? Plasmid containing PCR insert Plasmid alone No plasmid PCR insert All of the above
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
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transformed with which of the following could grow on the plate?
Plasmid containing PCR insert
Plasmid alone
No plasmid
PCR insert
All of the above"
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media. What was the next step in the laboratory procedures?
Random hexamer PCR
O Gel electrophoresis
OPCR of lacZ gene
Reverse transcription
O Extract RNA"
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