Rate in U [S] in mM No Inhibitor 0.5 1.0 2.0 4.0 8.0 16.0 32.0 64.0 0.22 0.41 0.73 1.12 1.57 2.01 2.3 2.5 3.0 mM Inhibitor Copy and paste the above data into Excel. Create an Eadie-Hofstee plot of the give dataset. 0.08 0.15 0.29 0.52 0.85 1.3 1.78 2.16 Calculate the Vmax, and Vmax/ Km parameters for both the uninhibited and inhibited trials (Inhibited parameters are marked with an apostrophe (')). Finally, determine the mechanism of inhibition. Note: give all parameters to two decimal places, and do not include the units. For th method of inhibition, state competitive, uncompetitive, or mixed as your answer. Vmax 1. Vmax= U-mM-1. Mechanism of inhibition = inhibition N U. Vmax/Km U. Vmax/Km= A U.mM
Rate in U [S] in mM No Inhibitor 0.5 1.0 2.0 4.0 8.0 16.0 32.0 64.0 0.22 0.41 0.73 1.12 1.57 2.01 2.3 2.5 3.0 mM Inhibitor Copy and paste the above data into Excel. Create an Eadie-Hofstee plot of the give dataset. 0.08 0.15 0.29 0.52 0.85 1.3 1.78 2.16 Calculate the Vmax, and Vmax/ Km parameters for both the uninhibited and inhibited trials (Inhibited parameters are marked with an apostrophe (')). Finally, determine the mechanism of inhibition. Note: give all parameters to two decimal places, and do not include the units. For th method of inhibition, state competitive, uncompetitive, or mixed as your answer. Vmax 1. Vmax= U-mM-1. Mechanism of inhibition = inhibition N U. Vmax/Km U. Vmax/Km= A U.mM
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
Related questions
Question
![Rate in U
[S] in mM No Inhibitor
0.5
1.0
2.0
4.0
8.0
16.0
32.0
64.0
0.22
0.41
0.73
1.12
1.57
2.01
2.3
2.5
3.0 mM
Inhibitor
Copy and paste the above data into Excel. Create an Eadie-Hofstee plot of the given
dataset.
0.08
0.15
0.29
0.52
0.85
1.3
1.78
2.16
Calculate the Vmax, and Vmax/ Km parameters for both the uninhibited and inhibited
trials (Inhibited parameters are marked with an apostrophe (')).
Finally, determine the mechanism of inhibition.
Note: give all parameters to two decimal places, and do not include the units. For the
method of inhibition, state competitive, uncompetitive, or mixed as your answer.
U. Vmax/Km-
A U. Vmax/Km=
Vmax
1. Vmax=
U-mM-1. Mechanism of inhibition =
inhibition
A/
A/
A/
U.mM
A](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F60f91569-8a7e-4f4e-b4e4-7ee2b7a34f80%2F053cfd93-5ad8-4dbd-a0b5-8d8f6cc06550%2F9yc3hkv_processed.png&w=3840&q=75)
Transcribed Image Text:Rate in U
[S] in mM No Inhibitor
0.5
1.0
2.0
4.0
8.0
16.0
32.0
64.0
0.22
0.41
0.73
1.12
1.57
2.01
2.3
2.5
3.0 mM
Inhibitor
Copy and paste the above data into Excel. Create an Eadie-Hofstee plot of the given
dataset.
0.08
0.15
0.29
0.52
0.85
1.3
1.78
2.16
Calculate the Vmax, and Vmax/ Km parameters for both the uninhibited and inhibited
trials (Inhibited parameters are marked with an apostrophe (')).
Finally, determine the mechanism of inhibition.
Note: give all parameters to two decimal places, and do not include the units. For the
method of inhibition, state competitive, uncompetitive, or mixed as your answer.
U. Vmax/Km-
A U. Vmax/Km=
Vmax
1. Vmax=
U-mM-1. Mechanism of inhibition =
inhibition
A/
A/
A/
U.mM
A
Expert Solution
This question has been solved!
Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.
Step by step
Solved in 3 steps with 6 images
Recommended textbooks for you
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
Biochemistry
Biochemistry
ISBN:
9781305961135
Author:
Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:
Cengage Learning
Biochemistry
Biochemistry
ISBN:
9781305577206
Author:
Reginald H. Garrett, Charles M. Grisham
Publisher:
Cengage Learning
Fundamentals of General, Organic, and Biological …
Biochemistry
ISBN:
9780134015187
Author:
John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson
Publisher:
PEARSON