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Rate in U [S] in mM No Inhibitor 0.5 1.0 2.0 4.0 8.0 16.0 32.0 64.0 0.22 0.41 0.73 1.12 1.57 2.01 2.3 2.5 3.0 mM Inhibitor Copy and paste the above data into Excel. Create an Eadie-Hofstee plot of the give dataset. 0.08 0.15 0.29 0.52 0.85 1.3 1.78 2.16 Calculate the Vmax, and Vmax/ Km parameters for both the uninhibited and inhibited trials (Inhibited parameters are marked with an apostrophe (')). Finally, determine the mechanism of inhibition. Note: give all parameters to two decimal places, and do not include the units. For th method of inhibition, state competitive, uncompetitive, or mixed as your answer. Vmax 1. Vmax= U-mM-1. Mechanism of inhibition = inhibition N U. Vmax/Km U. Vmax/Km= A U.mM

Rate in U [S] in mM No Inhibitor 0.5 1.0 2.0 4.0 8.0 16.0 32.0 64.0 0.22 0.41 0.73 1.12 1.57 2.01 2.3 2.5 3.0 mM Inhibitor Copy and paste the above data into Excel. Create an Eadie-Hofstee plot of the give dataset. 0.08 0.15 0.29 0.52 0.85 1.3 1.78 2.16 Calculate the Vmax, and Vmax/ Km parameters for both the uninhibited and inhibited trials (Inhibited parameters are marked with an apostrophe (')). Finally, determine the mechanism of inhibition. Note: give all parameters to two decimal places, and do not include the units. For th method of inhibition, state competitive, uncompetitive, or mixed as your answer. Vmax 1. Vmax= U-mM-1. Mechanism of inhibition = inhibition N U. Vmax/Km U. Vmax/Km= A U.mM

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Rate in U [S] in mM No Inhibitor 0.5 1.0 2.0 4.0 8.0 16.0 32.0 64.0 0.22 0.41 0.73 1.12 1.57 2.01 2.3 2.5 3.0 mM Inhibitor Copy and paste the above data into Excel. Create an Eadie-Hofstee plot of the given dataset. 0.08 0.15 0.29 0.52 0.85 1.3 1.78 2.16 Calculate the Vmax, and Vmax/ Km parameters for both the uninhibited and inhibited trials (Inhibited parameters are marked with an apostrophe (')). Finally, determine the mechanism of inhibition. Note: give all parameters to two decimal places, and do not include the units. For the method of inhibition, state competitive, uncompetitive, or mixed as your answer. U. Vmax/Km- A U. Vmax/Km= Vmax 1. Vmax= U-mM-1. Mechanism of inhibition = inhibition A/ A/ A/ U.mM A
Transcribed Image Text:Rate in U [S] in mM No Inhibitor 0.5 1.0 2.0 4.0 8.0 16.0 32.0 64.0 0.22 0.41 0.73 1.12 1.57 2.01 2.3 2.5 3.0 mM Inhibitor Copy and paste the above data into Excel. Create an Eadie-Hofstee plot of the given dataset. 0.08 0.15 0.29 0.52 0.85 1.3 1.78 2.16 Calculate the Vmax, and Vmax/ Km parameters for both the uninhibited and inhibited trials (Inhibited parameters are marked with an apostrophe (')). Finally, determine the mechanism of inhibition. Note: give all parameters to two decimal places, and do not include the units. For the method of inhibition, state competitive, uncompetitive, or mixed as your answer. U. Vmax/Km- A U. Vmax/Km= Vmax 1. Vmax= U-mM-1. Mechanism of inhibition = inhibition A/ A/ A/ U.mM A
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