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You would like to create a mouse model of the human disease Huntington's chorea, a dominant disease caused by a gain of function mutation in the human HD gene. Which of the following strategies would be suitable? Select all that apply 0 Use CRISPR-Cas9 to introduce frameshift mutations in the mouse HD gene; breed the resulting mutant mice to generate homozygous mutants 0 Introduce the wild type human HD allele as a transgene into mouse genome; select transgenics where human HD replaced the wild type mouse HD gene via homologous recombination Introduce the mutant human HD allele as a transgene into the mouse genome with transgene integration anywhere in the mouse genome Use CRISPR-Cas9 technology to cut the mouse HD gene; supply the mutant human HD allele as a repair template and select for transgenics where the the mutant human HD replaced the mouse HD gene

You would like to create a mouse model of the human disease Huntington's chorea, a dominant disease caused by a gain of function mutation in the human HD gene. Which of the following strategies would be suitable? Select all that apply 0 Use CRISPR-Cas9 to introduce frameshift mutations in the mouse HD gene; breed the resulting mutant mice to generate homozygous mutants 0 Introduce the wild type human HD allele as a transgene into mouse genome; select transgenics where human HD replaced the wild type mouse HD gene via homologous recombination Introduce the mutant human HD allele as a transgene into the mouse genome with transgene integration anywhere in the mouse genome Use CRISPR-Cas9 technology to cut the mouse HD gene; supply the mutant human HD allele as a repair template and select for transgenics where the the mutant human HD replaced the mouse HD gene

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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You would like to create a mouse model of the human disease Huntington's chorea, a dominant disease caused by a gain of function mutation in the human HD gene. Which of the following strategies would be suitable? Select all that apply Use CRISPR-Cas9 to introduce frameshift mutations in the mouse HD gene; breed the resulting mutant mice to generate homozygous mutants Introduce the wild type human HD allele as a transgene into mouse genome; select transgenics where human HD replaced the wild type mouse HD gene via homologous recombination Introduce the mutant human HD allele as a transgene into the mouse genome with transgene integration anywhere in the mouse genome Use CRISPR-Cas9 technology to cut the mouse HD gene; supply the mutant human HD allele as a repair template and select for transgenics where the the mutant human HD replaced the mouse HD gene
Transcribed Image Text:You would like to create a mouse model of the human disease Huntington's chorea, a dominant disease caused by a gain of function mutation in the human HD gene. Which of the following strategies would be suitable? Select all that apply Use CRISPR-Cas9 to introduce frameshift mutations in the mouse HD gene; breed the resulting mutant mice to generate homozygous mutants Introduce the wild type human HD allele as a transgene into mouse genome; select transgenics where human HD replaced the wild type mouse HD gene via homologous recombination Introduce the mutant human HD allele as a transgene into the mouse genome with transgene integration anywhere in the mouse genome Use CRISPR-Cas9 technology to cut the mouse HD gene; supply the mutant human HD allele as a repair template and select for transgenics where the the mutant human HD replaced the mouse HD gene
Part of the wild type sequence in a gene is AGGATCTTGGAC If this sequence is targeted by a guide RNA for Cas9 cleavage between the two adjacent T's, and no repair template is provided, which of the following alleles is expected to arise from NHEJ repair of the broken sequence? Select all that are in the range of expected outcomes for NHEJ repair. AGGATCTTGGAC AGGATCAAGGAC AGGATCGGAC AGGATCTTCTTGGAC
Transcribed Image Text:Part of the wild type sequence in a gene is AGGATCTTGGAC If this sequence is targeted by a guide RNA for Cas9 cleavage between the two adjacent T's, and no repair template is provided, which of the following alleles is expected to arise from NHEJ repair of the broken sequence? Select all that are in the range of expected outcomes for NHEJ repair. AGGATCTTGGAC AGGATCAAGGAC AGGATCGGAC AGGATCTTCTTGGAC
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