A molecule of composition5′-AAAAAAAAAAA-3′3′-TTTTTTTTTTTTT-5′is replicated in a solution containing unlabeled (notradioactive) GTP, CTP, and TTP plus adenine nucleoside triphosphate with all its phosphorus atoms in theform of the radioactive isotope 32P. Will both daughtermolecules be radioactive? Explain. Then repeat thequestion for the molecule5′-ATATATATATATAT-3′3′-TATATATATATATA-5′

Q: Contrast the resultant orientation of the macrodipoles of cytochrome b562 consisting of 4…

A: Helical structures like alpha-helix secondary structures in proteins have macrodipoles. Every…

Q: For each individual on gel, determine if the individual is homozygous for presence of the Alu…

A: In gel electrophoresis the fragments which are Small os size ( less base pairs) travel further in…

Q: A chromosome from a multicellular slime mold called purple oblex (Plasmodiophora oblexii) is 13%…

A: Chargaff’s rule is used in such calculation for determining the percentage of the DNA bases that…

Q: For each individual on each gel, determine if the individual is homozygous for presence of the Alu…

A: An Alu element is a short stretch of DNA originally characterized by the action of the Arthrobacter…

Q: Draw a haworth projection for a repeating disaccharide unit of pectin with one methylated and one…

A: A special type of fiber also known as polysaccharide constituted by long chains of sugars that can…

Q: In pyrimidine nucleosides the anti conformation predominates because of steric interactions with…

A: Pyrimidine nucleosides have anti-conformation as the oxygen molecule present on the second carbon is…

Q: Cells normally repair the lesion O-methylguanine (0°-meG) by directly transferring the methyl group…

A: Solution:This image presents a question about DNA repair, specifically the repair of…

Q: determine if the individual is homozygous for presence of the Alu insertion(++), heterozygous (+-),…

A: Each Alu sub family member assertion locus was assayed by PCR analysis. It is done by using…

Q: The data obtained from the experiment were fit to a single exponential function, which gave kobsd =…

A: The graph provided appears to represent a biochemical kinetic study, possibly relating to enzyme…

Q: starting and ending purity, t

A: Purification of a Recombinant Protein:This process involves the following steps-Cel harvesting- In…

Q: 5-Bromouridine is known to induce mutations in DNA. One of the characteristics of this compound is…

A: 5-Bromouridine is a uridine subordinate with a Bromo substituent at the fifth carbon. Br -Urd is…

Q: What two basepair changes can occur when G tautomerizes to the enol form? Recall that the…

A: Introduction: Spontaneous mutations result from numerous sources such as errors during DNA…

Q: 5-Bromouracil is a compound that is known to induce point mutations in DNA. 1) Out of A/C/G/T as…

A: DNA or deoxyribonucleic acid is a polymer of deoxyribonucleotides connected together via…

Q: For the following sequence, what is the approximate annealing temperature? 5'-AGCTACGATCAGGTCA-3'

A: PCR (Polymerase Chain Reaction) is divided into three main steps : Denaturation of DNA, Annealing of…

Q: Give two reasons to explain why a proline residue in the middle of an a helix is predicted to be…

A: Proline either breaks or kinks a helix.

Q: Why are the activated nucleotides in nucleic acid polymerization reactions 5’-triphosphates?

A: According to the question, we have to explain the reason for the activated nucleotides in nucleic…

Q: One of the useful rules of thumb that you may have learned in Biochemistry, is that a 50 g/ml…

A: In the above question, there should be a correction about the following information: “One of the…

Q: a) Q1 a. Justify why the base thyamine is petered over guanine in DNA while the reverse is…

A: Thymine helps stabilize nucleic acid structures. DNA is composed of two strands that twist upon each…

Q: 49 N), heavy parental DNA, and all nucleotides with 14N (another isotope of N) as "nucleotide supply…

A: We can use different shades or different colors represent a daughter strand of DNA with light N…

Q: РО P3mod 5' GAGTTTGATCMTGGCTCAG 3' .5' ATTAGATACCCTDGTAGTCC 3'

A: Answer well explained above

Q: 4. 5-methylcytosine results from which of the following eve

A: DNA methylation is a process in which the methyl group (CH3) is added to the nitrogenous base.…

Q: For the structure shown on the figure - qualitatively draw Ramachandran plot. Assume a mixture of…

A: Ramachandran plot is plot used to visualize energetically allowed regions for the peptide backbone…

Q: Ladder LS KT MV DY JY Ladder 850 bp 550 bp 11 1

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Q: The antibiotic cordycepin inhibits bacterial RNA synthesis. Solve, (a) Of which nucleoside is…

A: Cordycepin is an antibiotic, which also acts as an anti-inflammatory compound as well as…

Q: The 3′ → 5′ exonuclease activity of Pol I excises only unpaired 3′-terminal nucleotides from DNA,…

A: A technique discovered by Kary Mullis, polymerase chain reaction (PCR), is used to replicate the DNA…

Q: A mixture of four a-[*p]-labeled ribonucleoside triphosphates was added to permeabilized bacterial…

A: (a)By adding in excess unlabeled rNTPs, the labeled rNTPs that were added previously will stop being…

Q: 1. You order two primers from a company that synthesizes oligonucleotides. The primers have the…

A: Primers are short stretches of nucleotides that are used to initiate the synthesis of nucleic acids.…

Q: A protein has been sequenced after cleavage of disulfide bonds. The protein is known to contain 3…

A: There are four classes of biological macromolecules: Nucleic acids, Proteins, Lipids and…

Q: Rice genome contains 30% C on a molar basis. What are the mole percentages of A, G, and T?

A: In a double helical DNA, there are 4 nucleotides are found ATP, GTP, CTP, TTP. In this double…

Q: Rank the following in terms of predicted rates: the nucleation of an α helix;the nucleation of a…

A: Given: Need to justify  the nucleation of an α helix, parallel β sheet, antiparallel β sheet

Q: What would the order of migration be (bottom to top in the gel) in a SDS-PAGE for the following…

A: Introduction: Sodium dodecyl sulfate-polyacrylamide electrophoresis (SDS-PAGE) is an analytical…

Q: Disulfide bonds have been shown to stabilize proteins (i.e., make them less likely to unfold).…

A: Protein folding is driven by chemical and physical constraints. As a polypeptide chain folds into a…

Q: solution containing single stranded DNA with the sequence 5’ATGGTGCACCTGACTCCTGAGGAGAAGTCTNNNNN’3…

A: In this reaction, we have used dideoxyadenosine triphosphate. Hence, the reaction will stop at all…

Q: The octapeptide gly-cys-met-asn-lys-ala-tyr-gly was hydrolyzed consecutively by CNBr and then…

A: The octapeptide gly-cys-met-asn-lys-ala-tyr-gly was hydrolyzed consecutively by CNBr and then…

Q: If you want to purify a DNA-binding protein from a crude mixture of proteinsat pH 7, should you use…

A: Introduction: Proteins can be separated by ion-exchange chromatography based on their net charge.…

Q: The ends of eukaryotic chromosomes terminate in a G-rich singlestrandedoverhang that can fold up on…

A: Introduction: The compact form of DNA is known as chromosome. It is present inside the nucleus of…

Osmosis

In simple words, osmosis is the slow, gradual movement of a liquid through a membrane. But there are nuances to this process. For instance, there has to be an imbalance in the distribution of solute in the liquid on either side of the membrane, which has to be semipermeable. The liquid in the region with a lower solute concentration moves to the region with a higher concentration, thereby displacing the solution in the process. The objective is to equalize the concentration of molecules on both sides of the membrane; the displacement of liquid can be viewed as a byproduct of this equalization. Since there is no energy required to carry osmosis, it is considered a passive process.