A protein has been sequenced after cleavage of disulfide bonds. The protein is known to contain 3 Cys residues, located as shown below. Only one of the Cys has a free -SH group and the other two are involved in an -S-S- bond. CA N-terminus C-terminus The only methionine and the only aromatic amino acid (Phe) in this protein are in the positions indicated. Cleavage of the intact protein (i.e., with disulfide bonds intact) by chymotrypsin breaks into two proteins, but by cyanogen bromide does not.
A protein has been sequenced after cleavage of disulfide bonds. The protein is known to contain 3 Cys residues, located as shown below. Only one of the Cys has a free -SH group and the other two are involved in an -S-S- bond. CA N-terminus C-terminus The only methionine and the only aromatic amino acid (Phe) in this protein are in the positions indicated. Cleavage of the intact protein (i.e., with disulfide bonds intact) by chymotrypsin breaks into two proteins, but by cyanogen bromide does not.
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Question
X2.29
Transcribed Image Text:A protein has been sequenced after cleavage of disulfide bonds. The protein is known to contain 3 Cys residues, located as shown below. Only one of the Cys has a free -SH group and the other two are involved
in an -S-S- bond.
Part A
Where is the S―S bond (i.e., AB, BC, or AC)?
AC
AB
BC
Submit
A
-Cys
A
Met-
B
The only methionine and the only aromatic amino acid (Phe) in this protein are in the positions indicated. Cleavage of the intact protein (i.e., with disulfide bonds intact) by chymotrypsin breaks into two proteins, but
by cyanogen bromide does not.
Previous Answers
Phe
*N-terminus
C
C-terminus
X Incorrect; correct answer withheld by instructor
The disulfide is between Cys(B) and Cys(C). Any other disulfide would yield one peptide following treatment with chymostrypsin.
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