In order to simplify your analysis, rather than including an actual DNA sequence, I have simply included the gene of interest (shown with the blue line) with key regions, including the HindIII you do not need to restriction sites, indicated with labelled arrows. be concerned with the reading frame.

Q: The same restriction digestion experiment was repeated for EcoR1 on the same DNA molecule. However,…

A: It is given that the incubation period of the restriction digestion with EcoRI on the same DNA was…

Q: How  big (in base pairs)  is (are) the fragments generated by digesting the vector with EcoRI

A: Base pairs - there are four bases or nucleotides in DNA: ADENINE, CYTOSINE, GUANINE, and thymine.

Q: What is karyokinesis ? Explain with the help of diagram.

A: Cell division is defined as the process by which a single parent cell divides and produces two or…

Q: Restriction endonuclease digestion of a DNA sequence yielded fragments of the following sizes: 1.…

A: The objective of the question is to determine the order of DNA fragments after gel electrophoresis…

Q: Tm value, the GC-content of the selected DNA sequence and the absolute nucleotide composition of the…

A: DNA stands for deoxyribonucleic acid and it consists of 4 types of nitrogen bases, adenine, guanine,…

Q: With regards to this sequence below please answer this quistions 1) What is the format of the…

A: answer given below

Q: When you know the sequence of the gene for the ocular protein where would you get the DNA you need…

A: The cloning is the method of recombinant DNA technology in which a piece of known DNA sequence is…

Q: Solution B that was used during the plasmid isolation contains 0.2 M NaOH (see practical manual).…

A: INTRODUCTION E. coli E. coli  are small gram negative motile bacilli which mainly cause urinary…

Q: 6 - Restriction enzymes are used to Amplify the gene of interest D) O Ligase vector and gene of…

A: The restriction enzymes or restriction endonucleases are enzymes that cleave double-stranded DNA…

Q: Write the base sequence of the DNA template from which this RNA sequence was derived: UGUUACGGA. How…

A: Transcription  Cellular process in which RNA is synthesized using DNA as a template known as…

Q: Why is it possible to visualize a PCR product on an agarose gel even if the template genome is…

A: Gel electrophoresis is a technique used in the separation of macromolecules such as proteins and DNA…

Q: What is the significance of palindromic Restriction Endonuclease recognition and sequence overhangs…

A: Restriction endonucleases enzyme is also known as molecular scissors, which helps in cleavage of DNA…

Q: rationale for column-based DNA extraction and purification.

A: Introduction DNA extraction is a technique that involves methods and procedures to isolate DNA from…

Q: How many nucleotides are in the consensus coding sequence (CDS) of the KMT2D transcript?

A: A consensus coding sequence (CDS) is a method used in next-generation sequencing (NGS) to identify…

Q: We have two specific strains of E. coli that have shown horizontal gene transfer (HGT) when mixed.…

A: Introduction The non-sexual flow of genetic information between genomes is known as horizontal gene…

Q: What are the vectors used in rDNA technology? briefly explain each

A: rDNA technology or Recombinant DNA technology is a widely used technology with the advancement of…

Q: what type of gel(in terms of material) can be more suitable for the electrophoresis of 500-1000bp…

A: DNA tests are stacked into spaces toward one side of a gel, and an electric current is applied to…

Q: Explain the difference between sequencing by chain termination and sequencing by synthesis. Which…

A: Chain termination process employs dideoxynucleoside triphosphates (ddNTPs) lacking OH (hydroxyl)…

Q: Specify the method for Purification of DNA fragment after digestion.

A: Dna is the polymer formed by monomer units called nucleotides . The dna molecule when degraded…

Q: In the procedure called RNA sequencing (RNA-Seq), what type ofmolecule is actually sequenced?

A: Ribonucleic acid sequencing (RNA-seq) was developed by Michael Snyder and colleagues in 2008. It is…

Q: What are the reagents found in a restriction enzyme digestion buffer? What are the roles of these…

A: Restriction enzymes are enzymes that recognise and cleave DNA at or near specific DNA sequences.…

Q: Can DNA be isolated from beef? Discuss the process briefly

A: The purification of DNA from the collected sample of DNA with the help of the various methods and…

Q: See the attachment and answer the following parts of the question: A) If the binturong genome is…

A: Binturong is a eukaryote in which there is highly repetitive DNA sequence is present. The copies of…

Q: In the alkaline plasmid screen, what two things separate plasmid from chromosomal DNAs?

A: Following is the procedure for the separation of plasmid by the alkaline lysis method.

Q: Identify the basic steps (in right order) in AFLP marker analysis. Explain briefly how this marker…

A: Basic steps in AFLP marker analysis.

Q: --TGT(G/C)CAG------3'

A: SNV means single nucleotide variants on individual RNA molecules To identify genomic differences we…

Q: PCR-based assay was used for the detection of Candida albicans, please help me answer the following…

A: Note- we are supposed to answer only three subparts of a question according to our guidelines.…

Q: You have been given an unknown low weight polypeptide to sequence. Luckily, you have a plentiful…

A: Proteins are composed of amino acids, which are bound together by peptide linkage. Amino acids…

Q: For the following sequence, what is the Tm? 5'-AGCTACGATCAGGTCA-3'

A: Polymerase chain reaction (PCR) is a technique to amplify the sequence of DNA. A large quantity of…

Q: What is mass fragmentography? How is this performed?

A: Mass fragmentography is also known by the name mass fragmentometric analysis or mass fragmentography…

Q: Give three reasons why liposomes/nanoparticles are attractive drug delivery system for recombinant…

A: Introduction: Liposomes are a small-sized vesicle sharing structural and compositional similarity…

Q: What is a reason that a cut digest insert would not match up in length with a PCR insert in gel…

A: Electrophoresis describes the separation of charged particles and their migration in the influence…

Q: functionalization of a sensor array with DNA oligonucleotide capture probes for ribosomal RNA (rRNA)…

A: (B)The method which is based on sandwich hybridization of a capture and a detector probes with…

Q: Use the sequence provided and make use of figure 1 to determine what restriction enzyme uses the…

A: The lambda phage is a Escherichia coli bacteriophage. The virion DNA is linear and double-stranded…

Q: A 2.0kb bacterial plasmid ‘BS1030’ is digested with the restriction endonuclease Sau3A; the plasmid…

A: Plasmids are small, circular, double stranded, and extrachromosomal bacterial DNA. i.e. it is…

Q: Explain why the 16S rRNA gene sequencing is suitable for bacterial identification in general and why…

A: The Prokaryotes have a 70S type of ribosome (S=Svedberg units). The 70S ribosome is made up of a 50S…

Genome annotation

The technique of linking biological information to genome sequences is termed genome annotation. Gene annotation is the method of identifying gene locations and coding sections. It helps us understand what these genes are doing in the body through establishing structural characteristics and linking them to the actions of various proteins.