a.) wanting to clone gene Z into pVector, the gene is amplified by PCR and restriction sites are added to the flanking ends. without realizing that the antibotic resistant gen )tetR) had a Sal1 site, you decide to add EcoR1 and Sal1 recofnition sequencen into the 12-nucleotide primers.Write the sequence of the 2 primers, noting the 5' and 3' ends? You are cloning the human gene Z into a bacterial expression vector, pVector, which has atetracyclin resistant gene (tet). The maps of pVector and gene Z are shown below, with knownrestriction sites labelled. Restriction enzyme recognition sequences along with cut sites arealso noted. Assume that the distances between the restriction sites in the multiple cloning siteare negligible.Multiplecloning sitePromoterEcoR1Salt200 bpXho1700 bppVector2600bp600 bpEcoR1Sal1Xho1tetR5' - alA ATTC-3 5 - arcaAC-33 -CTTAAla -55' - CTCGAG-33-CAGCT|G - 5 3.GAGC T|C - 5Sal1ORI1100 bpSTARTSTOP5' - ATTCGGCGGATTTACGCGATATCG - 3'3'- ТАAGCCGCСТААGene ZATGCGCTATAGC - 5'800 bp

Plasmid vectors provide a simple way of cloning small DNA fragments in bacterial and simple…

Answered: a.) wanting to clone gene Z into…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

See similar textbooks

Related questions

Concept explainers

Question

a.) wanting to clone gene Z into pVector, the gene is amplified by PCR and restriction sites are added to the flanking ends. without realizing that the antibotic resistant gen )tetR) had a Sal1 site, you decide to add EcoR1 and Sal1 recofnition sequencen into the 12-nucleotide primers.

Write the sequence of the 2 primers, noting the 5' and 3' ends?

You are cloning the human gene Z into a bacterial expression vector, pVector, which has a
tetracyclin resistant gene (tet). The maps of pVector and gene Z are shown below, with known
restriction sites labelled. Restriction enzyme recognition sequences along with cut sites are
also noted. Assume that the distances between the restriction sites in the multiple cloning site
are negligible.
Multiple
cloning site
Promoter
EcoR1
Salt
200 bp
Xho1
700 bp
pVector
2600bp
600 bp
EcoR1
Sal1
Xho1
tetR
5' - alA ATTC-3 5 - arcaAC-3
3 -CTTAAla -5
5' - CTCGAG-3
3-CAGCT|G - 5 3.GAGC T|C - 5
Sal1
ORI
1100 bp
START
STOP
5' - ATTCGGCGGATT
TACGCGATATCG - 3'
3'- ТАAGCCGCСТАА
Gene Z
ATGCGCTATAGC - 5'
800 bp

Expert Solution

Step by step

Solved in 2 steps with 3 images

SEE SOLUTION Check out a sample Q&A here

Knowledge Booster

Learn more about

Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.

Similar questions