1. Use the equation of the linear regression line fitted to your standard curve and the migration of your DNA restriction fragments for enzyme mix A, B and C to interpolate the sizes for each DNA fragment. Show your working below. You can also upload a photo of your hand-written working out if preferred.
1. Use the equation of the linear regression line fitted to your standard curve and the migration of your DNA restriction fragments for enzyme mix A, B and C to interpolate the sizes for each DNA fragment. Show your working below. You can also upload a photo of your hand-written working out if preferred.
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
Related questions
Question
1. Use the equation of the linear regression line fitted to your standard curve and the migration of your DNA restriction fragments for enzyme mix A, B and C to interpolate the sizes for each DNA fragment.
Show your working below. You can also upload a photo of your hand-written working out if preferred.
Transcribed Image Text:D
1
2
3
4
5
5
7
B
9
D
1
2
3
4
5
5
7
3
9
D
1
2
3
4
5
A
Size of DNA marker (bp)
15000
10000
8000
7000
6000
5000
4000
3000
2000
1500
1000
850
650
500
400
300
200
100
B
log 10 of DNA marker
4.176091259
4
3.903089987
3.84509804
3.77815125
3.698970004
3.602059991
3.477121255
3.301029996
3.176091259
3
2.929418926
2.812913357
2.698970004
2.602059991;
2.477121255
2.301029996
2
C
Distance migrated by DNA marker from top of agaroses gel (mm)
molecular weight
4.5
2.5
2
1.5
1
0.5
0
Migration of the DNA markers (mm) vs
log10 molecular weight.
000 €
y=-0.0099x +4.5834
R²=0.9808
60
65
68
72
76
82
90
102
122
139
162.5
173.5
186
198
208.5
217
230
241
50
100
150
200
250
Distance migrated by DNA marker from top of agaroses gel (mm)
300
D
Transcribed Image Text:))
TTTTTTT
8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27
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