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Use the equation of the linear regression line fitted to your standard curve and the migration of your DNA restriction fragments for enzyme mix A, B and C to interpolate the sizes for each DNA fragment.  please i really need to understand this im not sure how to calculate for each enzyme

Use the equation of the linear regression line fitted to your standard curve and the migration of your DNA restriction fragments for enzyme mix A, B and C to interpolate the sizes for each DNA fragment.  please i really need to understand this im not sure how to calculate for each enzyme

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Use the equation of the linear regression line fitted to your standard curve and the migration of your DNA restriction fragments for enzyme mix A, B and C to interpolate the sizes for each DNA fragment. 

please i really need to understand this im not sure how to calculate for each enzyme

AB C 20 50 Distance 64 TO Distance on graph Migrated
Transcribed Image Text:AB C 20 50 Distance 64 TO Distance on graph Migrated
distance migrated (mm) DNA SIZE (BP) log10 (base size) 24 15000 4.176091259 25 10000 4 27 8000 3.903089987 28 7000 3.84509804 30 Migration of DNA markers 6000 3.77815125 33 5000 3.698970004 4.5 38 4000 3.602059991 4 42 3000 3.477121255 3.5 45 2000 3.301029996 3 49 1500 3.176091259 2.5 52 1000 3 y = -0.0286x + 4.6606., R2 = 0.9836 58 850 2.929418926 1.5 64 650 2.812913357 68 500 2.698970004 0.5 72 400 2.602059991 20 40 60 80 100 120 76 300 2.477121255 83 200 2.301029996 Distance Migrated 98 100 2 DNA SIZE (bp)
Transcribed Image Text:distance migrated (mm) DNA SIZE (BP) log10 (base size) 24 15000 4.176091259 25 10000 4 27 8000 3.903089987 28 7000 3.84509804 30 Migration of DNA markers 6000 3.77815125 33 5000 3.698970004 4.5 38 4000 3.602059991 4 42 3000 3.477121255 3.5 45 2000 3.301029996 3 49 1500 3.176091259 2.5 52 1000 3 y = -0.0286x + 4.6606., R2 = 0.9836 58 850 2.929418926 1.5 64 650 2.812913357 68 500 2.698970004 0.5 72 400 2.602059991 20 40 60 80 100 120 76 300 2.477121255 83 200 2.301029996 Distance Migrated 98 100 2 DNA SIZE (bp)
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