5. Below is an image of DNA sequenced using the Sanger sequencing method, where different nucleotides are labeled with different colors. Top of gel (shortest or longest DNA fragment?) a) Label the shortest and longest fragments on the gel by filling out the blank next to the image. ddGTP Labeled terminato Nucleotides used sequencing reactie ddCTP dLIPP ddATP b) Determine the sequence of the original gene (hint: remember that the sequence is complimentary to the original gene because it is copied by PCR (via base pairing) prior to loading the gel). Gel sequence:
5. Below is an image of DNA sequenced using the Sanger sequencing method, where different nucleotides are labeled with different colors. Top of gel (shortest or longest DNA fragment?) a) Label the shortest and longest fragments on the gel by filling out the blank next to the image. ddGTP Labeled terminato Nucleotides used sequencing reactie ddCTP dLIPP ddATP b) Determine the sequence of the original gene (hint: remember that the sequence is complimentary to the original gene because it is copied by PCR (via base pairing) prior to loading the gel). Gel sequence:
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Transcribed Image Text:5. Below is an image of DNA sequenced using the Sanger sequencing method, where different nucleotides are
labeled with different colors.
Top of gel
(shortest or longest
DNA fragment?)
a) Label the shortest and longest fragments on the gel by filling out the
blank next to the image.
ddGTP
Labeled terminator
ddCTP
Nucleotides used in
ddTTP
sequencing reaction
ddATP
b) Determine the sequence of the original gene (hint: remember that the
sequence is complimentary to the original gene because it is copied by PCR
(via base pairing) prior to loading the gel).
Gel sequence:
Original Gene:
+ (shortest or longest
DNA fragment?)
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