4. A linear fragment of DNA is exposed to a digest containing the individual restriction enzymes HindIII and SmaI and then with a combination of the two enzymes. According to the gel electrophoresis results, the fragments obtained are Linear DNA HindIII Smal cut with: HindIII + Smal DNA fragments (kb) 5.0 5.5 3.0 2.5 2.0 2.5 2.0 a. Draw a map of the restriction sites on this linear piece of DNA. b. The mixture of fragments produced by the combined enzymes was then digested with the enzyme EcoRI. The results are fragments that are 2.5 kb, 2.0 kb and 1.5 kb. Add the restriction site for EcoRI on the map you created above.

Q: 5) PCR primers have a melting temperature (Tm) at which they become dissociated from the template…

A: A primer is a short strand of nucleotide molecules that serves as an initial point for the synthesis…

Q: 4. You isolate DNA from 1 ml of a suspension of E. coli cells using the procedure outlined in your…

A: a. The dilution is 10:1 b. The original DNA concentration (without dilution) is 4400 ug/ml OR 4.4…

Q: During DNA replication, both positive and negative supercoiling is introduced in the DNA being…

A: Loosening up of the helix during Deoxyribose Nucleic Acid (DNA) replication (by the activity of…

Q: 36.  When choosing restriction enzymes for cloning a gene of interest into a plasmid, what is an…

A: Restriction enzyme:           An enzyme that cuts dsDNA into fragments after recognising specific…

Q: 51. In 1973, Cohen and Boyer created the first chimera by successfully incorporating the frog rRNA…

A: In 1973, Boyer and Cohen successfully recombined the two plasmids pSC 101 that contain gene…

Q: Explain how the SNP changes the recognition sequence for the restriction enzyme and affects its…

A: *Single nucleotide polymorphisms (SNPs) involve variation of a single base pair and or SNPsbin the…

Q: 2. Dr. Kim at Research Center performed shotgun Sanger sequencing on an unknown DNA sample, and…

A: In conventional sequencing method, in a reaction mixture,  single-stranded molecules of the DNA,…

Q: 4. A scientist cuts a large amount of produce a range of DNA pieces that are relatively similar in…

A: Agarose gel electrophoresis is a widely used technique for separating DNA fragments based on their…

Q: 5. You wish to map restriction sites in an unknown plasmid that has not been sequenced. You digest…

A: The process of fragmenting deoxyribonucleic acid strands with specific enzymes is restriction…

Q: This plasmid is digested with the enzymes listed below. Indicate how many fragments will be…

A:

Q: The restriction enzyme Xho I recognizes and cuts this sequence: 5-C/TCGAG-3. The restriction enzyme…

A: In molecular biology procedures, restriction enzymes are especially important for DNA cloning. They…

Q: BamHI Haelll Both 10 kb 9 kb 8 kb 5 kb 2 kb 1 kb Figure 2 How long is the original DNA molecule?

A: If it is a closed circular DNA, BamH1  cut it into three fragments of length 10kb 5kb 2kb

Q: 3. A plasmid was digested with the enzymes BglII, Nsil and XhoI. A single digest was performed with…

A: A plasmid is an extrachromosomal small DNA molecule present in a cell and is also physically…

Q: 1. Why do we need to check the isolated DNA for its quantity and quality? 2. The purity of DNA…

A: Deoxyribonucleic acid DNA isolation is referred to the extraction process of DNA from different…

Q: 24. The diagram below shows the map for EcoRI restriction sites around the BRCA1 gene for two…

A: Answer well explained above.

Q: 3 The restriction endonuclease PstI cuts DNA symmetrically on both strands at the СTGCAG sequence:…

A: Restriction endonucleases are the enzymes that are used to form cuts inside the DNA molecule. It is…

Q: Use the equation of the linear regression line fitted to your standard curve and the migration of…

A: Agarose gels are often run to check DNA properties in several molecular biology experiments. The…

Q: You have a DNA sequence of 1400 bp. To characterize it, you decide to make a restriction map.…

A: DNA is a nucleotide polymer present in living beings.

Q: There are various types of DNA-targeting drug, including DNA alkylating agents, DNA intercalators…

A: Mutations are DNA errors that occur during life. A mutation is defined as any alteration in the DNA…

Q: 1. Given the following restriction endonucleases and the sequences of their corresponding…

A: Prokaryotic cells are unicellular organisms that differ from multicellular organisms in composition…

Q: Match each statement with the best possible single answer from the list below. Terms may be used in…

A:

Q: 6. Below are several DNA sequences that are mutated compared with the wild-type sequence: 3'-TACT…

A: Wild type sequence:                           3' TACTGACTGACGATC 5' Complementary : 5'…

Q: X ↓ O 650 O 550 O 500 200 O 850 Smal ✓ O 1550 300 Msel 350 Probe Smal ↓ Caption: Restriction enzyme…

A: Restriction endonuclease are called molecular scissors because they cut at specialised DNA…

Q: 3. Primer Design You are analyzing the

A: Primer is a short stretch of oligonucleotide which is designed to copy the gene sequence of interest…

Q: 2. Your Genetics professor gives you and your classmate's information on a restriction digest. The…

A: The advantages of biotechnology for our society can be in drug manufacturing at a low cost using…

Q: 25. The restriction enzymes Kpnl and Acc65l recognize and cleave the same 6-bp sequence. You have a…

A: Restriction enzymes are the DNA digesting enzymes.

Q: 9. Estimate the sizes of the bands, in each of the lanes for the gel attached below. Then, for each…

A: A restriction endonuclease or restriction enzyme is a bacterial enzyme that cuts dsDNA into…

Q: 1. The polymerase chain reaction (PCR) is used by scientists to amplify DNA, particularly when the…

A: We are answering 1st question only. For the rest of the questions please repost. The polymerase…

Q: Dr. Doom has a DNA sequence of 2000 bp. Enzyme X and Enzyme Y restriction sites and their positions…

A: Restriction enzymes are the enzymes which cut DNA at particular site.

Q: 3. A linear DNA fragment is cleaved with one, two or three restriction enzymes to yield fragments as…

A: Restriction enzymes are those that cut the DNA at specific regions. Different restriction enzymes…

Q: 1. Use the equation of the linear regression line fitted to your standard curve and the migration of…

A: Gel electrophoresis is a technique used to separate DNA, RNA and protein molecules based on their…

Q: 19. You run a PCR of the above HBB sequence using the primers you designed. You receive a vector…

A: i) Gel Electrophoresis Bands:- The ladder serves as a reference for the sizes of the DNA fragments.-…

Q: fragments will be formed-a, b, and c. Which of the following gels produced by electrophoresis would…

A: A DNA segment is cut by two restriction enzymes and the three fragments are separated on an agarose…

Q: 1. a. Draw the primers for this longer strand (consider optimal length):…

A: Introduction  PCR is the revolutionized technique invented by Karry Mullis in 1983, for which he got…

Q: b. After molecular cloning and confirming you properly cloned stripes into the vector shown, you…

A: The goal of this study is to investigate the structure of the protein product STRIPES, which…

Q: A 2.0kb bacterial plasmid ‘BS1030’ is digested with the restriction endonuclease Sau3A; the plasmid…

A: Plasmids are small, circular, double stranded, and extrachromosomal bacterial DNA. i.e. it is…

Q: DNA ladder Sample digested with EcoRI negative electrode 23- This is the result of gel…

A: Introduction Gel electrophoresis is a technique that uses size and charge to separate and analyse…

Genome annotation

The technique of linking biological information to genome sequences is termed genome annotation. Gene annotation is the method of identifying gene locations and coding sections. It helps us understand what these genes are doing in the body through establishing structural characteristics and linking them to the actions of various proteins.