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4. A linear fragment of DNA is exposed to a digest containing the individual restriction enzymes HindIII and SmaI and then with a combination of the two enzymes. According to the gel electrophoresis results, the fragments obtained are Linear DNA HindIII Smal cut with: HindIII + Smal DNA fragments (kb) 5.0 5.5 3.0 2.5 2.0 2.5 2.0 a. Draw a map of the restriction sites on this linear piece of DNA. b. The mixture of fragments produced by the combined enzymes was then digested with the enzyme EcoRI. The results are fragments that are 2.5 kb, 2.0 kb and 1.5 kb. Add the restriction site for EcoRI on the map you created above.

4. A linear fragment of DNA is exposed to a digest containing the individual restriction enzymes HindIII and SmaI and then with a combination of the two enzymes. According to the gel electrophoresis results, the fragments obtained are Linear DNA HindIII Smal cut with: HindIII + Smal DNA fragments (kb) 5.0 5.5 3.0 2.5 2.0 2.5 2.0 a. Draw a map of the restriction sites on this linear piece of DNA. b. The mixture of fragments produced by the combined enzymes was then digested with the enzyme EcoRI. The results are fragments that are 2.5 kb, 2.0 kb and 1.5 kb. Add the restriction site for EcoRI on the map you created above.

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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4. A linear fragment of DNA is exposed to a digest containing the individual restriction enzymes HindIII and Smal and then with a combination of the two enzymes. According to the gel electrophoresis results, the fragments obtained are Linear DNA HindIII Smal cut with: DNA HindIII + Smal 5.0 fragments 2.5 (kb) 5.5 3.0 2.0 2.5 2.0 a. Draw a map of the restriction sites on this linear piece of DNA. b. The mixture of fragments produced by the combined enzymes was then digested with the enzyme EcoRI. The results are fragments that are 2.5 kb, 2.0 kb and 1.5 kb. Add the restriction site for EcoRI on the map you created above.
Transcribed Image Text:4. A linear fragment of DNA is exposed to a digest containing the individual restriction enzymes HindIII and Smal and then with a combination of the two enzymes. According to the gel electrophoresis results, the fragments obtained are Linear DNA HindIII Smal cut with: DNA HindIII + Smal 5.0 fragments 2.5 (kb) 5.5 3.0 2.0 2.5 2.0 a. Draw a map of the restriction sites on this linear piece of DNA. b. The mixture of fragments produced by the combined enzymes was then digested with the enzyme EcoRI. The results are fragments that are 2.5 kb, 2.0 kb and 1.5 kb. Add the restriction site for EcoRI on the map you created above.
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