5. A scientist was studying a fragment of eukaryotic DNA that she suspected was involved in a disease process. She isolated the fragment from the genomic DNA and then labeled the ends of the DNA with radioactive tags. This radioactively labeled fragment was then subjected to 3 restriction enzyme digest treatments: EcoRI single digest, BamHI single digest and EcoRI/BamHI double digest. The results are shown on the following table. The asterisk (*) indicated fragments containing the radioactive label. Restriction Enzyme Fragment Size (bp) 400, 200*, 100*, 50 350*, 250, 15О* ЕcoRI + BamНІ |250, 200*, 100*, 50 ВатHI Draw the restriction map for this DNA fragment.
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
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