4. A scientist cuts a large amount of produce a range of DNA pieces that are relatively similar in size. Scientists can vary the density of the agar gels by varying the amount of agar in the gel. Would she want to make an agarose gel with a higher or lower amount of agarose in order to resolve the DNA bands? A higher amount of agarose in the gel makes it stiffer. Explain.

Q: 16) In a DNA molecule, the phosphate group is attached to the 5' end of a nucleotide True or false…

A: The answer is true .…

Q: ing the gel picture of the DNA ladder, show where your digestion of the paper cutting model showing…

A: DNA is a double stranded biomolecule. The DNA is usually digested by endonucleases and exonucleases…

Q: 1.     You are having problems getting good transfection efficiency when you put your plasmid in e.…

A: Transfection is the process of deliberately introducing naked or purified nucleic acids into…

Q: labeled mitotic cells (%) 100- 75- 50 25 0 bod 0 5 10 15 20 25 30 time (hours) a) Would cells at all…

A: Introduction The cell cycle is the cascade of different events that leads to cell growth and…

Q: Using Beer's Law, please calculate the concentration of your sample if your absorbance reading at…

A: According to the Beer's Law which is also known as Beer-Lambert law states that the absorbance…

Q: 3. A plasmid was digested with the enzymes BglII, Nsil and XhoI. A single digest was performed with…

A: A plasmid is an extrachromosomal small DNA molecule present in a cell and is also physically…

Q: 9. You have two tubes, each containing a different DNA fragment. The fragments are the same…

A: Restriction enzymes are those which cut DNA at a specific location called as restriction site.

Q: DNA is charged and _fragments travel faster than positively, shorter, longer negatively, shorter,…

A: DNA is a polymer that is composed of two polynucleotide chains that coil around each other but…

Q: 1. The image shown in Figure 1 below represents a strand of DNA following replication. The black…

A: DNA repair mechanisms helps to repair many accidental lesions that occurs continuously in DNA.…

Q: Unknown 150 bp 250 bp 400 bp 700 bp mnipcrt pivot bio interactives GEL Change Gel 3

A: The restriction fragment length polymorphism technique (RFLP) "cuts" out genes which are likely to…

Q: 30.Match the definition with one of the choices on the right (can be used more than once) Place with…

A: DNA or deoxyribonucleic acid is the main molecule that is responsible for the purpose of storing…

Q: 26. You are a forensic scientist investigating a murder for the FBI. Foreign blood was found on the…

A: In biotechnology different techniques are developed for the determination of specific…

Q: Why do you increase the pH to denature the plasmid and chromosomal DNA during alkaline lysis rather…

A: In the alkaline lysis, we increase pH to denature the plasmid and chromosomal DNA( Deoxyribonucleic…

Q: 5- What’s the purpose of the DNA extraction? a- Force the cell to make more DNA b- Kill the cells.…

A:

Q: u transfer 0.2 ml of DNA solution from Tube A and place it into Tube B. How much total s present in…

A: C1V1=C2V2 is used to calculate an unknown quantity where two solutions/mixtures are proportional.…

Q: 6. You perform a restriction digest that should yield 1,400 bp, 1,080 bp, 1,000 bp, and 400 bp…

A: There are different biomolecules, including DNA, RNA, proteins, etc., are present, and these…

Q: 3. A long DNA will be divided into small fragments, and one of them will be attached to the bead and…

A: Ion torrent sequencing is the method of sequencing by signals of pH change voltage generation, which…

Q: 4.) Below is a replication fork. This is half of a replication bubble. 3' 3' a. Draw a circle to…

A: The process of copying and duplicating a DNA molecule called DNA replication..Helicase breaks double…

Q: where are the bacteria that did not take up the plasmid during the transformation

A: Introduction : The technique of horizontal gene transfer known as bacterial transformation allows…

Q: 4. A linear fragment of DNA is exposed to a digest containing the individual restriction enzymes…

A: For doing restriction mapping we need to arrange each fragments at it's proper position. The process…

Q: 1... Describe the different models for DNA replication (conservative, semi-conservative, dispersive)…

A: The DNA replication is the process by which new DNA is synthesized from the old DNA. In case of…

Q: 33.Choose the DNA nucleotide sequence that would be complementary to the following strand (note: pay…

A: DNA is  store house of genetic information. During S phase of cell cycle DNA make its copies to…

Q: 3. You isolate DNA from 1 ml of a suspension of E. coli cells using the procedure outlined in your…

A: a. 10 μl DNA solution was added to 490 μl buffer to make a total volume of 500 μl. Therefore the…

Q: 30. Decide whether the statement is true or false, and then explain why. Pulsed-field gel…

A: Gel electrophoresis is a scientific technique that is used to separate mixtures of DNA, RNA, or…

Q: 4. Draw and label a model that shows how complementary base-pairing is used to create a new strand…

A: DNA replication occurs within the cytoplasm of prokaryotic cells and nucleus of eukaryotic cells.…

Gene Interactions

When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.

Gene Expression

Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.