labeled mitotic cells (%) 100- 75- 50 25 0 bod 0 5 10 15 20 25 30 time (hours) a) Would cells at all phases of the cell cycle contain radioactive DNA immediately after the labeling procedure? Why? b) Why are there initially no mitotic cells that contain radioactive DNA? c) Explain the rise and fall and then rise again of the curve. d) Estimate the length of G2 for these cells e) Estimate the length of the total cell cycle forthese cells

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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6. Consider an experiment in which cells are radioactively labeled by this method for only a
short period of time (about 30 minutes). The radioactive thymidine medium is then replaced
with medium containing unlabeled thymidine, and the cells are grown for some additional
time. At different time points after replacement of the medium, cells are examined in a
microscope. The fraction of cells in mitosis (which can be easily recognized because their
chromosomes are condensed) that have radioactive DNA in their nuclei is then determined
and plotted as a function of time after the labeling with radioactive thymidine.
labeled mitotic cells (%)
100
75-
50
25
0 bod
0 5
10 15 20 25 30
time (hours)
a)
Would cells at all phases of the cell
cycle contain radioactive DNA immediately
after the labeling procedure? Why?
b)
Why are there initially no mitotic cells that
contain radioactive DNA?
c) Explain the rise and fall and then rise again of the curve.
d) Estimate the length of G2 for these cells
e) Estimate the length of the total cell cycle forthese cells
Transcribed Image Text:6. Consider an experiment in which cells are radioactively labeled by this method for only a short period of time (about 30 minutes). The radioactive thymidine medium is then replaced with medium containing unlabeled thymidine, and the cells are grown for some additional time. At different time points after replacement of the medium, cells are examined in a microscope. The fraction of cells in mitosis (which can be easily recognized because their chromosomes are condensed) that have radioactive DNA in their nuclei is then determined and plotted as a function of time after the labeling with radioactive thymidine. labeled mitotic cells (%) 100 75- 50 25 0 bod 0 5 10 15 20 25 30 time (hours) a) Would cells at all phases of the cell cycle contain radioactive DNA immediately after the labeling procedure? Why? b) Why are there initially no mitotic cells that contain radioactive DNA? c) Explain the rise and fall and then rise again of the curve. d) Estimate the length of G2 for these cells e) Estimate the length of the total cell cycle forthese cells
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