You are studying a new fungus, G. lucasus, and are interested the molecules that control the cell cycle of this new organism. You make some mutant cells and find an intriguing mutant that has two mutations - one in the force gene and one in the darkside gene. a. You perform propidium-iodide cell cycle analysis by flow cytometry on wild-type and the double mutant cells and your results are below. During what stage(s) of the cell cycle are the double mutant cells arresting their growth? Please briefly explain your answer. Wild-Type Cells # of cells 180 kDa 110 kDa 80 kDa b. In studying the gene products of the force and darkside genes, you determine that the force gene produces a polypeptide of 80 kDa, and the darkside gene produces a polypeptide of 42 kDa. You collect lysates from wild-type cells in G₁, S, or G₂/M phases of the cell cycle and perform a Western blot (immunoblot) with either an antibody against force or an antibody against darkside... only you forget to label your blots (rookie mistake!). 60 kDa 42 kDa 2 relative DNA content 30 kDa # of cells Double-Mutant Cells Gel #1 Wild-Type Cells in: Ladder G₁ SG₂/M 2 relative DNA content 180 kDa 110 kDa 80 kDa 60 kDa 42 kDa 30 kDa Gel #2 Wild-Type Cells in: Ladder G₁ S G₂/M i) Which gene (force or darkside) displays a pattern of gene expression similar to a cyclin-dependent kinase (Cdk)? Briefly explain your answer. Be sure to include in your answer which gel (#1 or #2) you are using to come to your conclusion. ii) Which gene (force or darkside) displays a pattern of gene expression similar to a cyclin? Briefly explain your answer. Be sure to include in your answer which gel (#1 or #2) you are using to come to your conclusion.
You are studying a new fungus, G. lucasus, and are interested the molecules that control the cell cycle of this new organism. You make some mutant cells and find an intriguing mutant that has two mutations - one in the force gene and one in the darkside gene. a. You perform propidium-iodide cell cycle analysis by flow cytometry on wild-type and the double mutant cells and your results are below. During what stage(s) of the cell cycle are the double mutant cells arresting their growth? Please briefly explain your answer. Wild-Type Cells # of cells 180 kDa 110 kDa 80 kDa b. In studying the gene products of the force and darkside genes, you determine that the force gene produces a polypeptide of 80 kDa, and the darkside gene produces a polypeptide of 42 kDa. You collect lysates from wild-type cells in G₁, S, or G₂/M phases of the cell cycle and perform a Western blot (immunoblot) with either an antibody against force or an antibody against darkside... only you forget to label your blots (rookie mistake!). 60 kDa 42 kDa 2 relative DNA content 30 kDa # of cells Double-Mutant Cells Gel #1 Wild-Type Cells in: Ladder G₁ SG₂/M 2 relative DNA content 180 kDa 110 kDa 80 kDa 60 kDa 42 kDa 30 kDa Gel #2 Wild-Type Cells in: Ladder G₁ S G₂/M i) Which gene (force or darkside) displays a pattern of gene expression similar to a cyclin-dependent kinase (Cdk)? Briefly explain your answer. Be sure to include in your answer which gel (#1 or #2) you are using to come to your conclusion. ii) Which gene (force or darkside) displays a pattern of gene expression similar to a cyclin? Briefly explain your answer. Be sure to include in your answer which gel (#1 or #2) you are using to come to your conclusion.
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
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Chapter1: The Human Body: An Orientation
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Question
Transcribed Image Text:You are studying a new fungus, G. lucasus, and are interested the molecules that control the cell cycle of this new organism. You make some mutant cells and find an intriguing mutant that
has two mutations - one in the force gene and one in the darkside gene.
a. You perform propidium-iodide cell cycle analysis by flow cytometry on wild-type and the double mutant cells and your results are below. During what stage(s) of the cell cycle are the
double mutant cells arresting their growth? Please briefly explain your answer.
# of cells
Wild-Type Cells
In
180 kDa
110 kDa
80 kDa
b. In studying the gene products of the force and darkside genes, you determine that the force gene produces a polypeptide of 80 kDa, and the darkside gene produces a polypeptide of 42
kDa. You collect lysates from wild-type cells in G₁, S, or G₂/M phases of the cell cycle and perform a Western blot (immunoblot) with either an antibody against force or an antibody against
darkside ... only you forget to label your blots (rookie mistake!).
60 kDa
42 kDa
relative DNA content
30 kDa
# of cells
Gel #1
Wild-Type Cells in:
S G₂/M
Ladder G₁
Double-Mutant Cells
relative DNA content
180 kDa
110 kDa
80 kDa
60 kDa
42 kDa
30 kDa
Ladder
Gel #2
Wild-Type Cells in:
G₁
S G₂/M
i) Which gene (force or darkside) displays a pattern of gene expression similar to a cyclin-dependent kinase (Cdk)? Briefly explain your answer. Be sure to include in your answer which
gel (#1 or #2) you are using to come to your conclusion.
ii) Which gene (force or darkside) displays a pattern of gene expression similar to a cyclin? Briefly explain your answer. Be sure to include in your answer which gel (#1 or #2) you are
using to come to your conclusion.
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