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If the stop nucleotides were not added prior to loading the samples in the gel, what would the results look like? AGC T AGCT AGCT AGC T

If the stop nucleotides were not added prior to loading the samples in the gel, what would the results look like? AGC T AGCT AGCT AGC T

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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**Question 15.** If the stop nucleotides were not added prior to loading the samples in the gel, what would the results look like? Below this question, there are four diagrams labeled a-d, each illustrating a different pattern of results in a gel electrophoresis experiment. Each diagram is a rectangular box divided into four columns labeled 'A', 'G', 'C', and 'T'. These labels correspond to the different nucleotides (adenine, guanine, cytosine, and thymine). - **Diagram (a)**: Displays multiple parallel lines distributed across all four columns, indicating the presence of several bands at different positions in the gel. - **Diagram (b)**: Shows a single band near the bottom of each column, indicating that all samples stopped at a similar point during the electrophoresis. - **Diagram (c)**: Displays a single band in the middle of each column, indicating a consistent stopping point in all samples at a mid-range position in the gel. - **Diagram (d)**: Shows a single band near the top of each column, indicating that all samples stopped at an early point during the electrophoresis process. **Answer Options:** a. Multiple bands across different positions in all columns. b. A single band near the bottom of the gel in all columns. c. A single band in the middle of the gel in all columns. d. A single band near the top of the gel in all columns.
Transcribed Image Text:**Question 15.** If the stop nucleotides were not added prior to loading the samples in the gel, what would the results look like? Below this question, there are four diagrams labeled a-d, each illustrating a different pattern of results in a gel electrophoresis experiment. Each diagram is a rectangular box divided into four columns labeled 'A', 'G', 'C', and 'T'. These labels correspond to the different nucleotides (adenine, guanine, cytosine, and thymine). - **Diagram (a)**: Displays multiple parallel lines distributed across all four columns, indicating the presence of several bands at different positions in the gel. - **Diagram (b)**: Shows a single band near the bottom of each column, indicating that all samples stopped at a similar point during the electrophoresis. - **Diagram (c)**: Displays a single band in the middle of each column, indicating a consistent stopping point in all samples at a mid-range position in the gel. - **Diagram (d)**: Shows a single band near the top of each column, indicating that all samples stopped at an early point during the electrophoresis process. **Answer Options:** a. Multiple bands across different positions in all columns. b. A single band near the bottom of the gel in all columns. c. A single band in the middle of the gel in all columns. d. A single band near the top of the gel in all columns.
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