8. Consider a relaxed, covalently-closed circular DNA plasmid that has 2080 basepairs with Wr = 0. An intercalator molecule is added, such that there is oneintercalator per 104 base pairs. The effect of the intercalator is to cause the twistbetween the base pairs that flank it to be reduced to zero. What will be the resultingchange in supercoiling state (i.e., the Linking Number) for the intercalator-boundDNA ?

Answered: 8. Consider a relaxed,…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

See similar textbooks

Related questions

Concept explainers

Question

8. Consider a relaxed, covalently-closed circular DNA plasmid that has 2080 base
pairs with Wr = 0. An intercalator molecule is added, such that there is one
intercalator per 104 base pairs. The effect of the intercalator is to cause the twist
between the base pairs that flank it to be reduced to zero. What will be the resulting
change in supercoiling state (i.e., the Linking Number) for the intercalator-bound
DNA ?

Expert Solution

Step 1: Introduction

The scenario introduces an intercalator molecule. Intercalators are molecules that may insert between bases in DNA, disrupting the regular spacing of base pairs and unwinding of the double helix. This particular intercalator reduces the twist between flanking base pairs to zero. The question then asks about the resulting change in the supercoiling state or linking number for the intercalator-bound DNA. This involves understanding how intercalation affects DNA supercoiling and calculating the change in linking number

Step by step

Solved in 3 steps

SEE SOLUTION Check out a sample Q&A here

Knowledge Booster

Learn more about

Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.

Similar questions