Briefly outline how MLPA probe-sets can simultaneously detect multiple targets using a single set of PCR primers.

Q: What is the functional difference between regular PCR and qPCCR?

A: PCR stands for a polymerase chain reaction. As the name suggests that polymerase chain reaction…

Q: List and briefly describe the function of 4 proteins used in DNA replication in a E. coli but NOT…

A: The process of copying a DNA molecule to produce its two identical copies is termed as the DNA…

Q: Describe the applications of PCR in identifi cation techniques.

A: The polymerase chain reaction is based on the utilization of the polymerase enzyme. It is studied…

Q: Describe how transposable elements are used as experimental tools.

A: Mutation is defined as sudden inheritable change that occurs in the DNA sequence. Transposon also…

Q: Describe the function of restriction enzymes and ligase in genetic recombination technology.

A: The branch of biology that deals with forming a recombinant DNA by joining the DNA segments from two…

Q: Explain the purpose of the antibiotic resistance gene in this experiment. Why is this genetic trait…

A: Bacteria are microorganism that most commonly occur in the soil, air, water and in adverse…

Q: Explain any two real-time PCR and their applications specifically

A: A polymerase chain reaction (PCR) refers to the molecular biological technique that facilitates the…

Q: What is a cloning vector? Give two examples of specific DNA molecules routinely used as cloning…

A: Genetic material is nothing but the sequence of nucleic acids which is called as DNA. It contains…

Q: When using a conventional plasmid cloning vector containing a b-galactosidase gene, it is possible…

A: The blue–white screen is a techinque that allows for the rapid and convenient detection of…

Q: Briefly describe two different methods for inserting foreign DNA into plasmids, giving the strengths…

A: The plasmid is typically a small circular deoxyribonucleic acid (DNA) strand in the cytoplasm of the…

Q: Site directed mutagenesis is used to: 1.determine the critical base per sequences in a Genome 2.…

A: DNA consists of double strands of sequentially arranged nucleotides. The nucleotide sequence present…

Q: Outline the method of real-time PCR, and discuss why it is used.

A: PCR which stands for polymerization chain reaction is an analytic technique which is used to amplify…

Q: Bacteriophage lambda is as one of the routinely used molecular cloning vectors, which alsoserved as…

A: Gene cloning is a process in which our gene of interest is located into the gene and copied (cloned)…

Microbial Taxonomy

Taxonomy is the science of naming, classifying, and describing organisms. The taxonomy will include all the plants, microorganisms, and animals on this planet. Carl Linnaeus coined the taxonomy term. He is known as the father of taxonomy. Microbial taxonomy is a criterion of grouping microorganisms into groups based on their similarities and past relatedness. Microorganisms belonging to the same group are differentiated from the others via grouping into categories. This differentiation makes the study of microorganisms easy. There are different types of microbial taxonomies like Numerical and Molecular taxonomy. The study of microorganisms is known as microbiology. Microbiology is a broad term that takes into account microbial cultures, taxonomic studies, evolutionary relationships. Microbial interaction and diversity are huge data that needs to be compiled under the subject of microbiology. Microbiology covers various organisms like bacteria, fungi, algae, plants, animals, etc.