1) Which primer could be used as the forward primer, for synthesizing DNA from left to right in the above diagram?  Enter the number of the primer here: 2) Which primer could be used as the reverse primer, for synthesizing DNA from right to left in the above diagram? Enter the number of the primer here: 3)  Will these primers be part of the final PCR product? a.No, neither primer will be incorporated into the PCR product. b.The forward primer but not the reverse primer will be incorporated into the PCR product. c.The reverse primer but not the forward primer will be incorporated into the PCR product. d.Yes, both primers will be incorporated into the PCR product.

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
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Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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1) Which primer could be used as the forward primer, for synthesizing DNA from left to right in the above diagram?  Enter the number of the primer here:

2) Which primer could be used as the reverse primer, for synthesizing DNA from right to left in the above diagram? Enter the number of the primer here:

3) 

Will these primers be part of the final PCR product?

a.No, neither primer will be incorporated into the PCR product.

b.The forward primer but not the reverse primer will be incorporated into the PCR product.

c.The reverse primer but not the forward primer will be incorporated into the PCR product.

d.Yes, both primers will be incorporated into the PCR product.

Please answer the next few questions based on this scenario: DNA sequences flanking a region you
wish to amplify are shown below:
5' CACGTAGAAAGCCAGTCCGCAGAAACGGTGCT...GATACAGAACGAATTGCTTGCAGGCATCTCA3'
3' GTGCATCTTTCGGTCAGGCGTCTTTGCCACGA...CTATGTCTTGCTTAACGAACGTCCGTAGAGT5'
Eight possible primer sequences are listed below.
#1 5' GTAGAAAGCCAGTCCGCAGAAACG 3'
3'
#2 5' GCAAAGACGCCTGACCGAAAGATG
CGTTTCTGCGGACTGGCTTTCTAC 3'
#3 5'
#4 5' CATCTTTCGGTCAGGCGTCTTTGC
3'
3'
#5 5' CAGAACGAATTGCTTGCAGGCATC
#6 5' CTACGGACGTTCGTTAAGCAAGAC
3'
#7 5' GATGCCTGCAAGCAATTCGTTCTG 3'
#8 5' GTCTTGCTTAACGAACGTCCGTAG 3'
Transcribed Image Text:Please answer the next few questions based on this scenario: DNA sequences flanking a region you wish to amplify are shown below: 5' CACGTAGAAAGCCAGTCCGCAGAAACGGTGCT...GATACAGAACGAATTGCTTGCAGGCATCTCA3' 3' GTGCATCTTTCGGTCAGGCGTCTTTGCCACGA...CTATGTCTTGCTTAACGAACGTCCGTAGAGT5' Eight possible primer sequences are listed below. #1 5' GTAGAAAGCCAGTCCGCAGAAACG 3' 3' #2 5' GCAAAGACGCCTGACCGAAAGATG CGTTTCTGCGGACTGGCTTTCTAC 3' #3 5' #4 5' CATCTTTCGGTCAGGCGTCTTTGC 3' 3' #5 5' CAGAACGAATTGCTTGCAGGCATC #6 5' CTACGGACGTTCGTTAAGCAAGAC 3' #7 5' GATGCCTGCAAGCAATTCGTTCTG 3' #8 5' GTCTTGCTTAACGAACGTCCGTAG 3'
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