Cause Would result in the following error Unbalanced nucleotide concentrations a) Sequence errors in the amplicon hon specfic NO amplication of template sequence emors in amplicahon ampl cahoN in unngsize ampicahoN Annealing temperature too low amplication Poor primer specificity (wrong sequence) Template DNA degraded Contamination of reaction tubes or solutions Lack of Mg2+ in reaction mixture Pick one cause and result pair and discuss in detail on the next page why one wouldy
Nucleotides
It is an organic molecule made up of three basic components- a nitrogenous base, phosphate,and pentose sugar. The nucleotides are important for metabolic reactions andthe formation of DNA (deoxyribonucleic acid) and RNA (ribonucleic acid).
Nucleic Acids
Nucleic acids are essential biomolecules present in prokaryotic and eukaryotic cells and viruses. They carry the genetic information for the synthesis of proteins and cellular replication. The nucleic acids are of two types: deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). The structure of all proteins and ultimately every biomolecule and cellular component is a product of information encoded in the sequence of nucleic acids. Parts of a DNA molecule containing the information needed to synthesize a protein or an RNA are genes. Nucleic acids can store and transmit genetic information from one generation to the next, fundamental to any life form.
Please help answer the starred question
![6. Application:
There are four possible observations that suggest an error occurred in a PCR reaction:
a) Sequence errors in the amplicon, b) the amplicon is the wrong size, c) there is no
amplicon, or d) there are multiple, non-specific amplicons.
Next to the possible cause, write which error is most likely associated with the cause (the
first one is done as an example):
Cause
Would result in the following error
Unbalanced nucleotide concentrations
a) Sequence errors in the amplicon
Annealing temperature too low
hon specfic
c amplication
Poor primer specificity (wrong sequence)
NO amplication of template
sequence emurs in amplicahw
ampl.caton in unngsize
o n
Template DNA degraded
Contamination of reaction tubes or solutions
Lack of Mg2+ in reaction mixture
amplicabon
UN
米
Pick one cause and result pair and discuss in detail on the next page why one wouldy
lead to the other:](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F15cf0284-ad7c-4d0c-a2cc-33136ddb3eaa%2F6baa496f-02a4-4b4d-a524-e45710c69791%2Fjux8jwu_processed.jpeg&w=3840&q=75)
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