1. Linear DNA and the pUC plasmid were both digested with Hind III and EcoRI and then put together in a ligation reaction. Assume complete digestion. What are the sizes of the 2 possible ligation products? Show your work. Hind II EcoRI BamH1 Xbol 150 bp 200 bp 400 bp 100 bp 200 bp MCS: EcoRI - 396 Amp Sacl - 402 pUC lacz Smal - 412 2686 bp Xbol - 423 Pstl - 435 Hindll - 447 2. Following the ligation performed in #1 above, suppose that you next do a transformation and plate the cells on media containing ampicillin and X-gal. Complete the following table to describe your anticipated results. Ampicillin Production of Color of cells Cells resistance? B-galactosidase? on the plate? Without plasmid NO NO NO Cells With nonrecombinant plasmid Yes Yes Blve With recombinant plasmid Yes Intact Plasmid recomoinon Plashid ampr amp (4) Taczt lacz 67 ()Pan Laboratory 6 Mini-Genome Sequencing Project Part 2

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