1. Linear DNA and the pUC plasmid were both digested with Hind III and EcoRI and then put together in a ligation reaction. Assume complete digestion. What are the sizes of the 2 possible ligation products? Show your work. Hind II EcoRI BamH1 Xbol 150 bp 200 bp 400 bp 100 bp 200 bp MCS: EcoRI - 396 Amp Sacl - 402 pUC lacz Smal - 412 2686 bp Xbol - 423 Pstl - 435 Hindll - 447 2. Following the ligation performed in #1 above, suppose that you next do a transformation and plate the cells on media containing ampicillin and X-gal. Complete the following table to describe your anticipated results. Ampicillin Production of Color of cells Cells resistance? B-galactosidase? on the plate? Without plasmid NO NO NO Cells With nonrecombinant plasmid Yes Yes Blve With recombinant plasmid Yes Intact Plasmid recomoinon Plashid ampr amp (4) Taczt lacz 67 ()Pan Laboratory 6 Mini-Genome Sequencing Project Part 2

Q: i am confused on which question you answer

A: 26. Cognitive health for a 80 years old patient should include: Cognitive health is just one feature…

Q: QUESTION 7 Explain the steps in the healing of a bone fracture. v 3 (12pt) ттT Arial

A: Introduction: Bones comprise two different types of tissues, namely, spongy bone tissue and compact…

Q: Von Gierkes disease is the deficiency of Glucose -6- phosphate O True O False is the precursor for…

A:

Q: Question 10 The signs of internal bleeding include all of the following except: a. black,…

A: Signs of internal bleeding are given :-

Q: Photophosphoryation and the Calvin Cycle Q2.2- What happens to the net glyceraldehyde-3P produced by…

A: Introduction: A series of chemical processes that take place as a byproduct of photosynthesis and…

Q: What does phase 3 represent in the attached figure?

A: Neurons are structural and functional units of the nervous system. Neurons control the activity of…

Q: How do you do this assignment?

A: You have to answer the fill in the blanks. The answers are in the snowballs jumbled. Name the…

Q: Salma Rick Layla Adie Mac A Rh A Rh Rh Rh Rh B B B The blood type for Salma is The blood type for…

A: The blood type for Salma is A positive The blood type for Rick is AB negative  The blood type for…

Q: Question 63 During a depressive episode, what changes occur in the amygdala? Question 63 options:…

A: The correct option will be the first one- New disorganized neuronal pathways are formed.

Q: Question 12

A: COMPLETE BLOOD COUNT CBC (Complete blood count) is a blood test that is used to determine the…

Q: Which is the correct answer. I don't see it in your response.

A: The propagation of nerve impulses from one neuron to the next neuron occur at the synapse. The axon…

Q: Question 2 When heating substances in a test tube, be sure the open end points towards O 1. yourself…

A:

Q: how long is the wait for the answer

A: Absorption is the process whereby molecules are taken up by cells from the extracellular fluid and…

Q: Pain is considered a common for many conditions. Sign Symptorm Risk factor Differential diagnosis

A: Pain is an unpleasant sensory or emotional experience, caused by an illness or injury.  Types Acute…

Q: _cells stimulate both arms of the immune response (one arm is self and the other arm is foreign)

A: According to guideliness we have to answer the first question only so please kindly post the 2nd…

Q: To whom should he speak regarding what currently occurs when an employee is ill or when there is a…

A: In an organization, two people play a major role, the employer and the employee. In any form of the…

Q: What is the difference between DM and DI?

A: Hello there ,  Antidiuretic hormone (ADH), commonly referred to as vasopressin, levels are what…

Q: Specific prevention. Exists or not. Briefly explain why.

A: A virus is defined as an infectious microorganism made up of a protein-coated segment of nucleic…

Q: Difficulty extending the knee can result from damage to the

A: The knee is the body's largest joint and one of the most vulnerable to injury. Bones, cartilage,…

Q: An excitatory neurotransmitter secreted by motor neurons innervating skeletal muscle is O…

A: Motor neurons are the part of central nervous system. These neurons are responsible for transmitting…

Q: There is more than one right answer

A: During exercise, physical activity increases and different voluntary movements are produced.…

Q: Help please let me answer

A: We know that Lymphatic vessels are a network of capillaries and a huge network of tubes that move…

Q: Question no 1 ASAP please

A: We know that the Nervous system is a type of body system which generally deals with control and…

Q: Ms. S complained of gas distension of the abdomen on the 2nd postoperative day. The Nurse would. O…

A: Gas distension or abdominal bloating is the condition that develops in the abdomen when it is filled…

Q: 器 6 *器 S5 * ** The karyotype pictured here represents A. a normal male B. a normal female OC.a Down…

A: The representation of all the chromosomes of the cell nucleus of males or females is representation…

Q: why 7

A: Xylem transport is the process by which water and dissolved minerals are transported from the roots…

Q: Can Chris be Frank’s father?  Why or why not?

A: DNA (deoxyribonucleic acid) fingerprinting is a technique mostly used to determine the biological…

Q: ffnn is O homozygous O heterozygous O homozygous dominant

A: Trait are characteristic features that are unique to a particular individual . As per the question…

Q: QUESTION 11 What is the purpose of homogenization? OIt increases the absorption of DNA molecules.…

A: Please note that keeping up with policy at Bartleby and in order to provide accurate answers with…

Q: Experiment B: Community ecologists study communities in different locations, and ask why the number…

A: Biodiversity refers to the biological species present in an area.