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The map of plasmid pUC19 is shown below.  The restriction site coordinate is the position of the 5’base on the top strand of each site sequence.  The restriction enzyme sites are in bold type if there is only one site in pUC19. Please list the fragments in order of size, largest to smallest, which will result from a complete digestion by the restriction enzyme PvuII. Please list the fragments in order of size, largest to smallest, which will result from a complete digestion by the restriction enzyme DrdI.

The map of plasmid pUC19 is shown below.  The restriction site coordinate is the position of the 5’base on the top strand of each site sequence.  The restriction enzyme sites are in bold type if there is only one site in pUC19. Please list the fragments in order of size, largest to smallest, which will result from a complete digestion by the restriction enzyme PvuII. Please list the fragments in order of size, largest to smallest, which will result from a complete digestion by the restriction enzyme DrdI.

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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The map of plasmid pUC19 is shown below.  The restriction site coordinate is the position of the 5’base on the top strand of each site sequence.  The restriction enzyme sites are in bold type if there is only one site in pUC19.

  1. Please list the fragments in order of size, largest to smallest, which will result from a complete digestion by the restriction enzyme PvuII.
  2. Please list the fragments in order of size, largest to smallest, which will result from a complete digestion by the restriction enzyme DrdI. 
自动保存 O Individual Assignment 3 关闭 开始 插入 绘图 设计 布局 引用 邮件 审阅 视图 EndNote X9 图片格式 9告诉我 它共享 口批注 国压缩图片 可组合v T||高度: 4.4" 更改图片v 删除 背景 艺术效果 透明度 图片边框图片效果 颜色 位置 自动换行 前移一层 后移一层 选择 窗格 对齐 格式 窗格 更正 裁剪 Alt 文本 シ旋转v 宽度: 5.85" 重置图片v 1 2 3 4 7 T-Pspl, PI-Scel, Paci, Paek7I, PrlFl, Pmll, PpuMI, PshAI, Psil, PspOMI, Sacll, SanDI(x), SexAl, Sfil, SgrAl, Srfl(x), Stul, Styl, Swal, Tlil, nl, Xhol not available from NEB DrdI 91 BsmBI 51 BSTAPI 179 BsmBI 2683 Ndel 183 Eco01091 2674 KasI - Narl - Sfol 235 AatII - Zral 2617 Вс\VI 2542 SspI 2501 Bgll 245 FspI 256 3. Pvul 276 Pvull 306 Bmrl 364 AclI 2297 ВсеАl 387 lacza Apol - EcoRI 396 Banll - SacI - Eco53KI 402 XmnI 2294 Аcс651 - Крnl 408 Aval - BsoBI - Smal - TspMI - Xmal 412 BcgI 2215 MCS Scal 2177 BamHI 417 Xbal 423 Pvul 2066 Accl - HinclI - Sall 429 Avall 2059 BspMI - BfuAI 433 Sbfi 434 PUC19 2,686 bp BsrDI 1935 Pstl 435 AclI 1924 Sphl 441 HindIII 447 FspI 1919 PvulL 628 Avall 1837 Tfil 641 NmeAlII 1822 BglI 1813 Bpml 1784 BsaXI 659 BSPQI - SapI 683 BsrFI 1779 Tfil 781 Bsal 1766 AflIII - Pcil 806 BsrDI 1753 DrdI 908 BmrI 1744 ori AhdI 1694 BCİVI 1015 BseYI 1110 AlwNI 1217 ВсеАl 1292 第1页,共2页 236 个字 区 英语(美国) 专注 220% + < >
Transcribed Image Text:自动保存 O Individual Assignment 3 关闭 开始 插入 绘图 设计 布局 引用 邮件 审阅 视图 EndNote X9 图片格式 9告诉我 它共享 口批注 国压缩图片 可组合v T||高度: 4.4" 更改图片v 删除 背景 艺术效果 透明度 图片边框图片效果 颜色 位置 自动换行 前移一层 后移一层 选择 窗格 对齐 格式 窗格 更正 裁剪 Alt 文本 シ旋转v 宽度: 5.85" 重置图片v 1 2 3 4 7 T-Pspl, PI-Scel, Paci, Paek7I, PrlFl, Pmll, PpuMI, PshAI, Psil, PspOMI, Sacll, SanDI(x), SexAl, Sfil, SgrAl, Srfl(x), Stul, Styl, Swal, Tlil, nl, Xhol not available from NEB DrdI 91 BsmBI 51 BSTAPI 179 BsmBI 2683 Ndel 183 Eco01091 2674 KasI - Narl - Sfol 235 AatII - Zral 2617 Вс\VI 2542 SspI 2501 Bgll 245 FspI 256 3. Pvul 276 Pvull 306 Bmrl 364 AclI 2297 ВсеАl 387 lacza Apol - EcoRI 396 Banll - SacI - Eco53KI 402 XmnI 2294 Аcс651 - Крnl 408 Aval - BsoBI - Smal - TspMI - Xmal 412 BcgI 2215 MCS Scal 2177 BamHI 417 Xbal 423 Pvul 2066 Accl - HinclI - Sall 429 Avall 2059 BspMI - BfuAI 433 Sbfi 434 PUC19 2,686 bp BsrDI 1935 Pstl 435 AclI 1924 Sphl 441 HindIII 447 FspI 1919 PvulL 628 Avall 1837 Tfil 641 NmeAlII 1822 BglI 1813 Bpml 1784 BsaXI 659 BSPQI - SapI 683 BsrFI 1779 Tfil 781 Bsal 1766 AflIII - Pcil 806 BsrDI 1753 DrdI 908 BmrI 1744 ori AhdI 1694 BCİVI 1015 BseYI 1110 AlwNI 1217 ВсеАl 1292 第1页,共2页 236 个字 区 英语(美国) 专注 220% + < >
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