Using the plasmid map of pBCH2.0 calculate the length of the shortest DNA fragment if this plasmid was digested with the restriction enzymes PstI and EcoRV.
Gene Interactions
When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.
Gene Expression
Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.
Using the plasmid map of pBCH2.0
calculate the length of the shortest DNA fragment if this plasmid was digested with the restriction enzymes PstI and EcoRV.
![You utilised two plasmids in this practical, pOTC and pOTC-A. Plasmids
are often represented using plasmid maps like the one below. This map
shows the positions of recognition sites for a number of restriction
enzymes (ECORV, Pstl, Xbal etc).
Pstl
EcoRV
(6789),
(623)
Хbal
1282)
EcoRI (1538)
BamHI (1545)
Kpnl (1613)
Hindll (1653)
(1691)
Pvull
(6103)
PBCH2.0
Sall
(7693 bp)
Pstl
(2401)
Bstl
Хbal
(4469)
(3188)](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F5c798406-758b-4899-956e-fb2266d888ea%2F4478b857-0a02-43f4-a982-87aa3019373f%2Ff9wtx79_processed.png&w=3840&q=75)
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