Using the plasmid map of pBCH2.0 calculate the length of the shortest DNA fragment if this plasmid was digested with the restriction enzymes PstI and EcoRV.

Human Anatomy & Physiology (11th Edition)
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Author:Elaine N. Marieb, Katja N. Hoehn
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Chapter1: The Human Body: An Orientation
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Using the plasmid map of pBCH2.0

calculate the length of the shortest DNA fragment if this plasmid was digested with the restriction enzymes PstI and EcoRV. 

You utilised two plasmids in this practical, pOTC and pOTC-A. Plasmids
are often represented using plasmid maps like the one below. This map
shows the positions of recognition sites for a number of restriction
enzymes (ECORV, Pstl, Xbal etc).
Pstl
EcoRV
(6789),
(623)
Хbal
1282)
EcoRI (1538)
BamHI (1545)
Kpnl (1613)
Hindll (1653)
(1691)
Pvull
(6103)
PBCH2.0
Sall
(7693 bp)
Pstl
(2401)
Bstl
Хbal
(4469)
(3188)
Transcribed Image Text:You utilised two plasmids in this practical, pOTC and pOTC-A. Plasmids are often represented using plasmid maps like the one below. This map shows the positions of recognition sites for a number of restriction enzymes (ECORV, Pstl, Xbal etc). Pstl EcoRV (6789), (623) Хbal 1282) EcoRI (1538) BamHI (1545) Kpnl (1613) Hindll (1653) (1691) Pvull (6103) PBCH2.0 Sall (7693 bp) Pstl (2401) Bstl Хbal (4469) (3188)
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