2. Use the plasmid to answer the following questions: [6T] a) Digest the plasmid with EcoRI only and then both EcoRI and BamHi together. Fill in the table to show the fragment sizes produced. [3] EcoRI only EcoRI and BamHI together Fragmen t sizes produced EcoRI b) In the space below, sketch the gel that would be produced by these two digestions to show how the fragments would separate. (Write the fragment length beside each of the bands on the gel). [3] BamHI 5050 bp BamHI 0/6400 bp 3100 bp 830 bp EcoRI
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
How do you answer these two questions?
![2. Use the plasmid to answer the following questions : [6T]
a) Digest the plasmid with EcoRI only and then both
EcoRI and BamHi together. Fill in the table to show the
fragment sizes produced. [3]
EcoRI only
EcoRI and
BamHI together
Fragmen
t sizes
produced
EcoRI
b) In the space below, sketch the gel that would be produced by these two digestions to show how
the fragments would separate. (Write the fragment length beside each of the bands on the gel). [3]
BamHI
5050 bp
BamHI
0/6400 bp
3100 bp
830 bp
EcoRI](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F7ac578d8-3df5-41c9-9b88-e192ad787771%2F74432b09-969e-474b-9005-58c14c5cc6fa%2Fqj1zt9_processed.jpeg&w=3840&q=75)
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