A research group discovers a new version of happyase, which they call happyase*, that catalyzes the chemical reaction: HAPPY.-SAD The researchers begin to characterize the enzyme. The researchers determined the kcat of the enzyme to be 555 s1. In a separate experiment with [EJ at 3.2 nM and (HAPPY) at 55 µM, the researchers find that Vo is equal to 220 nM s1. What is the measured Km of happyase* for its substrate HAPPY? Make sure to include units in the answer.

Biochemistry
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ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
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Chapter1: Biochemistry: An Evolving Science
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A research group discovers a new version of happyase, which they call happyase*, that catalyzes the chemical reaction:
HAPPY.--SAD
The researchers begin to characterize the enzyme. The researchers determined the kcat of the enzyme to be 555 s1. In a separate experiment with [EJ at
3.2 nM and (HAPPY) at 55 µM, the researchers find that Vo is equal to 220 nM s-1. What is the measured Km of happyase* for its substrate HAPPY? Make
sure to include units in the answer.
Transcribed Image Text:A research group discovers a new version of happyase, which they call happyase*, that catalyzes the chemical reaction: HAPPY.--SAD The researchers begin to characterize the enzyme. The researchers determined the kcat of the enzyme to be 555 s1. In a separate experiment with [EJ at 3.2 nM and (HAPPY) at 55 µM, the researchers find that Vo is equal to 220 nM s-1. What is the measured Km of happyase* for its substrate HAPPY? Make sure to include units in the answer.
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