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6-31 You do an enzyme kinetic experiment and calculate a Vmax of 100 If each assay used 0.1 mL of an μmol of product per minute. enzyme solution that had a concentration of 02 mg/mL, what would be the turnover number if the enzyme had a molecular weight of 128000 g/mole?

6-31 You do an enzyme kinetic experiment and calculate a Vmax of 100 If each assay used 0.1 mL of an μmol of product per minute. enzyme solution that had a concentration of 02 mg/mL, what would be the turnover number if the enzyme had a molecular weight of 128000 g/mole?

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
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**Enzyme Kinetics Problems** **Problem 6-31:** You perform an enzyme kinetic experiment and calculate a \( V_{\text{max}} \) of 100 µmol of product per minute. If each assay used 0.1 mL of an enzyme solution with a concentration of 0.2 mg/mL, what would the turnover number be if the enzyme had a molecular weight of 128,000 g/mole? **Problem 6-32:** The enzyme D-amino acid oxidase has a very high turnover number because D-amino acids are potentially toxic. The \( K_M \) for this enzyme ranges from 1 to 2 mM for aromatic amino acids, and from 15 to 20 mM for amino acids like serine, alanine, and acidic amino acids. Which of these amino acids are the preferred substrates for the enzyme? **Graph/Diagram Explanation:** There are no graphs or diagrams present in this text. The focus is on the analysis and calculations related to enzyme kinetics and substrate specificity.
Transcribed Image Text:**Enzyme Kinetics Problems** **Problem 6-31:** You perform an enzyme kinetic experiment and calculate a \( V_{\text{max}} \) of 100 µmol of product per minute. If each assay used 0.1 mL of an enzyme solution with a concentration of 0.2 mg/mL, what would the turnover number be if the enzyme had a molecular weight of 128,000 g/mole? **Problem 6-32:** The enzyme D-amino acid oxidase has a very high turnover number because D-amino acids are potentially toxic. The \( K_M \) for this enzyme ranges from 1 to 2 mM for aromatic amino acids, and from 15 to 20 mM for amino acids like serine, alanine, and acidic amino acids. Which of these amino acids are the preferred substrates for the enzyme? **Graph/Diagram Explanation:** There are no graphs or diagrams present in this text. The focus is on the analysis and calculations related to enzyme kinetics and substrate specificity.
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