You fuse the DNA encoding GFP (green fluorescent protein) to the C terminus of the PKK gene. You also create the following GFP-PKK variants -GFP Wild- type: N- Signal sequence Transmembrane domain Kinase domain Variant 1: N- -GFP Transmembrane domain Kinase domain Variant 2: N- GFP Signal sequence Transmembrane domain Variant 3: N- GFP Signal sequence Kinase domain Variant 4: N- -GFP Kinase domain If you examine wild-type PKK trafficking in a eukaryotic cell, which of the following are true? Select all that apply
You fuse the DNA encoding GFP (green fluorescent protein) to the C terminus of the PKK gene. You also create the following GFP-PKK variants -GFP Wild- type: N- Signal sequence Transmembrane domain Kinase domain Variant 1: N- -GFP Transmembrane domain Kinase domain Variant 2: N- GFP Signal sequence Transmembrane domain Variant 3: N- GFP Signal sequence Kinase domain Variant 4: N- -GFP Kinase domain If you examine wild-type PKK trafficking in a eukaryotic cell, which of the following are true? Select all that apply
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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
Transcribed Image Text:You are studying a eukaryotic protein PKK in the lab.
You fuse the DNA encoding GFP (green fluorescent protein) to the C terminus of the
PKK gene.
You also create the following GFP-PKK variants
-GFP
Wild- type: N-
Signal sequence Transmembrane domain
Kinase domain
Variant 1:
N-
GFP
Transmembrane domain
Kinase domain
Variant 2:
N-
-GFP
Signal sequence Transmembrane domain
Variant 3: N-
-GFP
Signal sequence Kinase domain
Variant 4:
N-
-GFP
Kinase domain
If you examine wild-type PKK trafficking in a eukaryotic cell, which of the following
are true?
Select all that apply
This fusion protein will be trafficked to the nucleus
This fusion protein is a cell membrane protein
This fusion protein will be trafficked to the Endoplasmic Reticulum
This fusion protein is a nuclear protein
Expert Solution

Step 1
Answer :-
- This fusion protein is a cell membrane protein.
- This fusion protein is a nuclear protein.
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