You fuse the DNA encoding GFP (green fluorescent protein) to the C terminus of the PKK gene. You also create the following GFP-PKK variants -GFP Wild- type: N- Signal sequence Transmembrane domain Kinase domain Variant 1: N- -GFP Transmembrane domain Kinase domain Variant 2: N- GFP Signal sequence Transmembrane domain Variant 3: N- GFP Signal sequence Kinase domain Variant 4: N- -GFP Kinase domain If you examine wild-type PKK trafficking in a eukaryotic cell, which of the following are true? Select all that apply

Human Anatomy & Physiology (11th Edition)
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Author:Elaine N. Marieb, Katja N. Hoehn
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You are studying a eukaryotic protein PKK in the lab.
You fuse the DNA encoding GFP (green fluorescent protein) to the C terminus of the
PKK gene.
You also create the following GFP-PKK variants
-GFP
Wild- type: N-
Signal sequence Transmembrane domain
Kinase domain
Variant 1:
N-
GFP
Transmembrane domain
Kinase domain
Variant 2:
N-
-GFP
Signal sequence Transmembrane domain
Variant 3: N-
-GFP
Signal sequence Kinase domain
Variant 4:
N-
-GFP
Kinase domain
If you examine wild-type PKK trafficking in a eukaryotic cell, which of the following
are true?
Select all that apply
This fusion protein will be trafficked to the nucleus
This fusion protein is a cell membrane protein
This fusion protein will be trafficked to the Endoplasmic Reticulum
This fusion protein is a nuclear protein
Transcribed Image Text:You are studying a eukaryotic protein PKK in the lab. You fuse the DNA encoding GFP (green fluorescent protein) to the C terminus of the PKK gene. You also create the following GFP-PKK variants -GFP Wild- type: N- Signal sequence Transmembrane domain Kinase domain Variant 1: N- GFP Transmembrane domain Kinase domain Variant 2: N- -GFP Signal sequence Transmembrane domain Variant 3: N- -GFP Signal sequence Kinase domain Variant 4: N- -GFP Kinase domain If you examine wild-type PKK trafficking in a eukaryotic cell, which of the following are true? Select all that apply This fusion protein will be trafficked to the nucleus This fusion protein is a cell membrane protein This fusion protein will be trafficked to the Endoplasmic Reticulum This fusion protein is a nuclear protein
Expert Solution
Step 1

Answer :- 

- This fusion protein is a cell membrane protein.

- This fusion protein is a nuclear protein.

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