Using baker's yeast it is possible to generate point mutations that destroy the kinase activity of CDK9/P-TEFB protein kinase. When genome-wide ChIP studies using anti-phosphorylated CTD antibodies and RNA-seq analysis of the mRNA purified from these cells were performed it was found that RNA Polymerase II was present in the 5' region of the coding regions of most protein coding genes, but mRNA levels were reduced for all genes tested. The cells that had this mutation were very sick and the mutation caused many of the cells to die. The abundance of mature mRNAs was lower than in non-mutant cells, and often lower than the levels of their corresponding pre-mRNA. Some of the mature mRNAs had short poly A tails and some were detected with none at all. Based on this information: Why are the poly A tails affected in this mutant?
Gene Interactions
When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.
Gene Expression
Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.
Using baker's yeast it is possible to generate point mutations that destroy the kinase activity of CDK9/P-TEFB protein kinase. When genome-wide ChIP studies using anti-phosphorylated CTD antibodies and RNA-seq analysis of the mRNA purified from these cells were performed it was found that RNA Polymerase II was present in the 5' region of the coding regions of most protein coding genes, but mRNA levels were reduced for all genes tested. The cells that had this mutation were very sick and the mutation caused many of the cells to die. The abundance of mature mRNAs was lower than in non-mutant cells, and often lower than the levels of their corresponding pre-mRNA. Some of the mature mRNAs had short poly A tails and some were detected with none at all. Based on this information: Why are the poly A tails affected in this mutant?
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