Notch is an important transmembrane receptor that is present on the plasma membrane. Curiously, it possesses a proteolytic cleavage site and an NLS in its intracellular domain. In a protein fragment complementation experiment the Notch receptor was fused to the N-terminal portion of GFP on its intracellular domain. Another protein that is known to act in the nucleus to mediate the Notch signal following its activation was fused to the C-terminal region of GFP. When both are expressed in the same cell, GFP is observed in the nucleus. In 3-4 sentences provide an explanation that would account for the expression of GFP exclusively in the nucleus.

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Osmosis

In simple words, osmosis is the slow, gradual movement of a liquid through a membrane. But there are nuances to this process. For instance, there has to be an imbalance in the distribution of solute in the liquid on either side of the membrane, which has to be semipermeable. The liquid in the region with a lower solute concentration moves to the region with a higher concentration, thereby displacing the solution in the process. The objective is to equalize the concentration of molecules on both sides of the membrane; the displacement of liquid can be viewed as a byproduct of this equalization. Since there is no energy required to carry osmosis, it is considered a passive process.