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You are studying a eukaryotic protein PKK in the lab. You fuse the DNA encoding GFP (green fluorescent protein) to the C terminus of the PKK gene. You also create the following GFP-PKK variants Wild- type: N- -GFP Signal sequence Transmembrane domain Kinase domain Variant 1: N- GFP Transmembrane domain Kinase domain Variant 2: N- -GFP Signal sequence Transmembrane domain Variant 3: N- -GFP Signal sequence Kinase domain Variant 4: N- GFP Kinase domain If you examine PKK variant 4 trafficking in a eukaryotic cell, which of the following are true? Select all that apply This fusion protein will be made as a cytosolic protein This fusion protein will not function like the wild-type protein This fusion protein will traffic to the cell membrane This fusion protein will not traffic to the cell membrane

You are studying a eukaryotic protein PKK in the lab. You fuse the DNA encoding GFP (green fluorescent protein) to the C terminus of the PKK gene. You also create the following GFP-PKK variants Wild- type: N- -GFP Signal sequence Transmembrane domain Kinase domain Variant 1: N- GFP Transmembrane domain Kinase domain Variant 2: N- -GFP Signal sequence Transmembrane domain Variant 3: N- -GFP Signal sequence Kinase domain Variant 4: N- GFP Kinase domain If you examine PKK variant 4 trafficking in a eukaryotic cell, which of the following are true? Select all that apply This fusion protein will be made as a cytosolic protein This fusion protein will not function like the wild-type protein This fusion protein will traffic to the cell membrane This fusion protein will not traffic to the cell membrane

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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You are studying a eukaryotic protein PKK in the lab. You fuse the DNA encoding GFP (green fluorescent protein) to the C terminus of the PKK gene. You also create the following GFP-PKK variants Wild- type: N- -GFP Signal sequence Transmembrane domain Kinase domain Variant 1: N- -GFP Transmembrane domain Kinase domain Variant 2: N- GFP Signal sequence Transmembrane domain Variant 3: N- GFP Signal sequence Kinase domain Variant 4: -GFP Kinase domain If you examine PKK variant 4 trafficking in a eukaryotic cell, which of the following are true? Select all that apply This fusion protein will be made as a cytosolic protein This fusion protein will not function like the wild-type protein This fusion protein will traffic to the cell membrane This fusion protein will not traffic to the cell membrane
Transcribed Image Text:You are studying a eukaryotic protein PKK in the lab. You fuse the DNA encoding GFP (green fluorescent protein) to the C terminus of the PKK gene. You also create the following GFP-PKK variants Wild- type: N- -GFP Signal sequence Transmembrane domain Kinase domain Variant 1: N- -GFP Transmembrane domain Kinase domain Variant 2: N- GFP Signal sequence Transmembrane domain Variant 3: N- GFP Signal sequence Kinase domain Variant 4: -GFP Kinase domain If you examine PKK variant 4 trafficking in a eukaryotic cell, which of the following are true? Select all that apply This fusion protein will be made as a cytosolic protein This fusion protein will not function like the wild-type protein This fusion protein will traffic to the cell membrane This fusion protein will not traffic to the cell membrane
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