FFFN.∙NSOH₂N-`Np-AKTAKTp-GSK3BGSK33p-p70S6Kp70S6Kp-4EBP14EBP1GAPDH Drug X shown below is a kinase inhibitor used to treat multiple types of breast cancer. Breast cancer cells are treated withthe drug and after 8 hr are lysed and the presence of various proteins is shown by a band on a western blot. The presenceof the phosphorylated form of the protein is shown by the presence of a band when probed with antibodies that recognizethe phosphorylated form of protein and are indicated by a p- in front of protein name (e.g. p-AKT). Based on thisinformation, which kinase is likely the target and is inhibited byJustify your answer in 3-4 sentences.Drug X.FFFN.-NSIZH₂N`NNo Drug(+) Drugp-AKTAKTp-GSK3BGSK3Bp-p70S6Kp70S6K

Answered: Drug X shown below is a kinase…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Related questions

Question

F
FFN.
∙N
S
O
H₂N-
`N
p-AKT
AKT
p-GSK3B
GSK33
p-p70S6K
p70S6K
p-4EBP1
4EBP1
GAPDH

Drug X shown below is a kinase inhibitor used to treat multiple types of breast cancer. Breast cancer cells are treated with
the drug and after 8 hr are lysed and the presence of various proteins is shown by a band on a western blot. The presence
of the phosphorylated form of the protein is shown by the presence of a band when probed with antibodies that recognize
the phosphorylated form of protein and are indicated by a p- in front of protein name (e.g. p-AKT). Based on this
information, which kinase is likely the target and is inhibited by
Justify your answer in 3-4 sentences.
Drug X.
F
FF
N.
-N
S
IZ
H₂N
`N
No Drug
(+) Drug
p-AKT
AKT
p-GSK3B
GSK3B
p-p70S6K
p70S6K

Expert Solution

Step 1

As given in the question, Drug X is a kinase inhibitor used to treat multiple types of breast cancer.

Kinases are enzyme which phosphorylates the target protein. Inhibition of kinase will inhibit the phosphorylation of target protein.

As can be seen from the given image, treatment with Drug X inhibits the phosphorylated form of Akt (Protein Kinase B), GSK3β (Glycogen Synthase Kinase 3β), p70S6K (p70S6 kinase) and 4EBP1 (Eukaryotic translation initiation factor 4E-binding protein 1). All these molecules are downstream of a common kinase which is inhibited by the drug X.