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A colleague has successfully purified the enzyme sphingomyelinase from bovine brain. She provides you with the following incomplete purification table: Specific Activity (nmol/min) (nmol/min/mg) (%) Purification Factor (-fold) Total Total Purification Step Activity Yield Protein (mg) 100 1 Whole homogenate 40-60% (NH4);SO4 CM-sepharose ConA-sepharose Gel filtration Isoelectric focusing 88,625 5,685 925 544 51,700 32,067 29,500 31,680 1,855 420 5 0.03 (A) (B) (C) Per your colleague's request, you agree to calculate: (A) the final specific activity, (B) the final % yield, and (C) the final fold-purification. - x x x x *xx x x

A colleague has successfully purified the enzyme sphingomyelinase from bovine brain. She provides you with the following incomplete purification table: Specific Activity (nmol/min) (nmol/min/mg) (%) Purification Factor (-fold) Total Total Purification Step Activity Yield Protein (mg) 100 1 Whole homogenate 40-60% (NH4);SO4 CM-sepharose ConA-sepharose Gel filtration Isoelectric focusing 88,625 5,685 925 544 51,700 32,067 29,500 31,680 1,855 420 5 0.03 (A) (B) (C) Per your colleague's request, you agree to calculate: (A) the final specific activity, (B) the final % yield, and (C) the final fold-purification. - x x x x *xx x x

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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A colleague has successfully purified the enzyme sphingomyelinase from bovine brain. She provides you with the following incomplete purification table: Specific Activity (nmol/min) (nmol/min/mg) (%) Purification Factor (-fold) Total Total Purification Step Activity Yield Protein (mg) 100 1 Whole homogenate 40-60% (NH4);SO4 CM-sepharose ConA-sepharose Gel filtration Isoelectric focusing 88,625 5,685 925 544 51,700 32,067 29,500 31,680 1,855 420 5 0.03 (A) (B) (C) Per your colleague's request, you agree to calculate: (A) the final specific activity, (B) the final % yield, and (C) the final fold-purification. - x x x x *xx x x
Transcribed Image Text:A colleague has successfully purified the enzyme sphingomyelinase from bovine brain. She provides you with the following incomplete purification table: Specific Activity (nmol/min) (nmol/min/mg) (%) Purification Factor (-fold) Total Total Purification Step Activity Yield Protein (mg) 100 1 Whole homogenate 40-60% (NH4);SO4 CM-sepharose ConA-sepharose Gel filtration Isoelectric focusing 88,625 5,685 925 544 51,700 32,067 29,500 31,680 1,855 420 5 0.03 (A) (B) (C) Per your colleague's request, you agree to calculate: (A) the final specific activity, (B) the final % yield, and (C) the final fold-purification. - x x x x *xx x x
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