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An experiment was carried out to measure the reaction rate of hydrolysis of acetylcholme (substrate) with serum enzymes (Eadie, 1949). In the experiment, two experiments were conducted, namely experiment 1 without using a prostigmine inhibitor and experiment 2 using a prostigmine inhibitor at 1.5 x 10^-7 mol/l. the data obtained are:     a. Is prostigmine competitive or noncompetitive inhibitor? b. determine the value of km and rmax for the two experiments, compare

An experiment was carried out to measure the reaction rate of hydrolysis of acetylcholme (substrate) with serum enzymes (Eadie, 1949). In the experiment, two experiments were conducted, namely experiment 1 without using a prostigmine inhibitor and experiment 2 using a prostigmine inhibitor at 1.5 x 10^-7 mol/l. the data obtained are:     a. Is prostigmine competitive or noncompetitive inhibitor? b. determine the value of km and rmax for the two experiments, compare

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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An experiment was carried out to measure the reaction rate of hydrolysis of acetylcholme (substrate) with serum enzymes (Eadie, 1949). In the experiment, two experiments were conducted, namely experiment 1 without using a prostigmine inhibitor and experiment 2 using a prostigmine inhibitor at 1.5 x 10^-7 mol/l. the data obtained are:

 

 

a. Is prostigmine competitive or noncompetitive inhibitor?

b. determine the value of km and rmax for the two experiments, compare

Substrate Concentration (mol/l) 0.0032 0.0049 0.0062 0.0080 0.0095 Initial Reaction Rate (mol/l min) Absence of Presence of Prostigmine Prostigmine 0.111 0.059 0.148 0.071 0.143 0.091 0.166 0.111 0.200 0.125
Transcribed Image Text:Substrate Concentration (mol/l) 0.0032 0.0049 0.0062 0.0080 0.0095 Initial Reaction Rate (mol/l min) Absence of Presence of Prostigmine Prostigmine 0.111 0.059 0.148 0.071 0.143 0.091 0.166 0.111 0.200 0.125
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