The drug troglitazone was used to treat diabetes but was withdrawn from the market when patients who took the drug suffered from severe side effects. The data below show the activity of an enzyme in the steroid biosynthetic pathway in the presence and absence of 10 µM troglitazone. [S] (µM) v0 (pmol min-1) Create a plot of 1/v0 vs. 1/[S]. Calculate KM and vmax in presence and absence of inhibitor. What type of inhibitor is troglitazone? Clearly support your answer.
The drug troglitazone was used to treat diabetes but was withdrawn from the market when patients who took the drug suffered from severe side effects. The data below show the activity of an enzyme in the steroid biosynthetic pathway in the presence and absence of 10 µM troglitazone. [S] (µM) v0 (pmol min-1) Create a plot of 1/v0 vs. 1/[S]. Calculate KM and vmax in presence and absence of inhibitor. What type of inhibitor is troglitazone? Clearly support your answer.
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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The drug troglitazone was used to treat diabetes but was withdrawn from the market when patients
who took the drug suffered from severe side effects. The data below show the activity of an enzyme
in the steroid biosynthetic pathway in the presence and absence of 10 µM troglitazone.
[S] (µM) v0 (pmol min-1)
Create a plot of 1/v0 vs. 1/[S]. Calculate KM and vmax in presence and absence of inhibitor. What
type of inhibitor is troglitazone? Clearly support your answer.
![### Enzyme Kinetics Table
This table presents the initial rate of reaction, \( v_0 \) (in pmol min\(^{-1}\)), for an enzyme-catalyzed reaction at various substrate concentrations \([S]\) (in \(\mu M\)), both in the absence and presence of troglitazone.
| \([S]\) (\(\mu M\)) | \( v_0 \) without troglitazone | \( v_0 \) with troglitazone |
|---------------------|-----------------------------|----------------------------|
| 0.50 | 0.00272 | 0.0015 |
| 1.00 | 0.00457 | 0.0026 |
| 2.00 | 0.00672 | 0.0044 |
| 4.00 | 0.00769 | 0.0067 |
### Explanation
- **Substrate Concentration \([S]\)**: Represents the concentration of substrate in micromolar (\(\mu M\)).
- **Initial Reaction Rate \( v_0 \)**: Indicates the rate of reaction measured in picomoles per minute (pmol min\(^{-1}\)).
### Observations
- **Without Troglitazone**: The initial reaction rate \( v_0 \) increases with the substrate concentration \([S]\).
- **With Troglitazone**: The initial reaction rate \( v_0 \) is lower at every substrate concentration tested, suggesting that troglitazone inhibits the reaction.
This data is useful for understanding how troglitazone affects enzyme activity and can be applied in studies related to drug interactions and enzyme kinetics.](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F321fd000-515a-4cf5-ac03-99096af58e6a%2F57b59f66-0a6e-433d-b067-a4cc5fdb66e8%2Ferdf1rd_processed.jpeg&w=3840&q=75)
Transcribed Image Text:### Enzyme Kinetics Table
This table presents the initial rate of reaction, \( v_0 \) (in pmol min\(^{-1}\)), for an enzyme-catalyzed reaction at various substrate concentrations \([S]\) (in \(\mu M\)), both in the absence and presence of troglitazone.
| \([S]\) (\(\mu M\)) | \( v_0 \) without troglitazone | \( v_0 \) with troglitazone |
|---------------------|-----------------------------|----------------------------|
| 0.50 | 0.00272 | 0.0015 |
| 1.00 | 0.00457 | 0.0026 |
| 2.00 | 0.00672 | 0.0044 |
| 4.00 | 0.00769 | 0.0067 |
### Explanation
- **Substrate Concentration \([S]\)**: Represents the concentration of substrate in micromolar (\(\mu M\)).
- **Initial Reaction Rate \( v_0 \)**: Indicates the rate of reaction measured in picomoles per minute (pmol min\(^{-1}\)).
### Observations
- **Without Troglitazone**: The initial reaction rate \( v_0 \) increases with the substrate concentration \([S]\).
- **With Troglitazone**: The initial reaction rate \( v_0 \) is lower at every substrate concentration tested, suggesting that troglitazone inhibits the reaction.
This data is useful for understanding how troglitazone affects enzyme activity and can be applied in studies related to drug interactions and enzyme kinetics.
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