TABLE 8.5 Michaelis–Menten parameters for selected enzymes, arranged in order of increasing efficiency as measured by kear/KM Enzyme Reaction Catalyzed KM(mol/L) Kear(s ") Kcat/Km[ (mol/L)-1s1] Chymotrypsin H,0 Ac-Phe-Ala Ac-Phe + Ala 1.5 x 10-2 0.14 9.3 H,0 Phe-Gly Phe + Gly 3 x 10-4 Pepsin 0.5 1.7 х 103 Tyrosyl-TRNA synthetase Tyroslne + TRNA → tyrosyl-TRNA 9 x 10-4 7.6 8.4 x 103 Cytldine 2', 3' cycllc phosphate H,0 + cytldine 3'- phosphate Ribonuclease 7.9 x 10-3 7.9 x 102 1.0 x 105 Carbonic anhydrase HCO, + H* H,0 + CO2 2.6 x 10-2 4 x 105 1.5 x 107 H,O Fumarate malate Fumarase 5 x 10-6 8 x 102 1.6 x 108

Biochemistry
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ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
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Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
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(a) If the total enzyme concentration  1 nmol/L, how many molecules of substrate can a molecule of enzyme process in each minute?
(b) Calculate kcat/KM for the enzyme reaction. Is this a fairly efficient enzyme? (as shown.)

TABLE 8.5 Michaelis–Menten parameters for selected enzymes, arranged in order of increasing efficiency as measured by kear/KM
Enzyme
Reaction Catalyzed
KM(mol/L)
Kear(s ")
Kcat/Km[ (mol/L)-1s1]
Chymotrypsin
H,0
Ac-Phe-Ala Ac-Phe + Ala
1.5 x 10-2
0.14
9.3
H,0
Phe-Gly Phe + Gly
3 x 10-4
Pepsin
0.5
1.7 х 103
Tyrosyl-TRNA
synthetase
Tyroslne + TRNA → tyrosyl-TRNA
9 x 10-4
7.6
8.4 x 103
Cytldine 2', 3'
cycllc phosphate
H,0
+ cytldine 3'-
phosphate
Ribonuclease
7.9 x 10-3
7.9 x 102
1.0 x 105
Carbonic anhydrase
HCO, + H*
H,0 + CO2
2.6 x 10-2
4 x 105
1.5 x 107
H,O
Fumarate malate
Fumarase
5 x 10-6
8 x 102
1.6 x 108
Transcribed Image Text:TABLE 8.5 Michaelis–Menten parameters for selected enzymes, arranged in order of increasing efficiency as measured by kear/KM Enzyme Reaction Catalyzed KM(mol/L) Kear(s ") Kcat/Km[ (mol/L)-1s1] Chymotrypsin H,0 Ac-Phe-Ala Ac-Phe + Ala 1.5 x 10-2 0.14 9.3 H,0 Phe-Gly Phe + Gly 3 x 10-4 Pepsin 0.5 1.7 х 103 Tyrosyl-TRNA synthetase Tyroslne + TRNA → tyrosyl-TRNA 9 x 10-4 7.6 8.4 x 103 Cytldine 2', 3' cycllc phosphate H,0 + cytldine 3'- phosphate Ribonuclease 7.9 x 10-3 7.9 x 102 1.0 x 105 Carbonic anhydrase HCO, + H* H,0 + CO2 2.6 x 10-2 4 x 105 1.5 x 107 H,O Fumarate malate Fumarase 5 x 10-6 8 x 102 1.6 x 108
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