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a) Based on the data shown in the image, what are the Km and Vmax for the enzyme with  L-DOPA and D-DOPA? Show any relevant analyses or calculatins you did to determine these  values. ( HINT a graph might be helpful here! )   b) Based on your answer to part a, briefly describe how the kinetics of the enzyme  differs for the two substrates. Which Substrate has better binding affinity to the enzyme

a) Based on the data shown in the image, what are the Km and Vmax for the enzyme with  L-DOPA and D-DOPA? Show any relevant analyses or calculatins you did to determine these  values. ( HINT a graph might be helpful here! )   b) Based on your answer to part a, briefly describe how the kinetics of the enzyme  differs for the two substrates. Which Substrate has better binding affinity to the enzyme

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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a) Based on the data shown in the image, what are the Km and Vmax for the enzyme with 
L-DOPA and D-DOPA? Show any relevant analyses or calculatins you did to determine these 
values. ( HINT a graph might be helpful here! )  
b) Based on your answer to part a, briefly describe how the kinetics of the enzyme 
differs for the two substrates. Which Substrate has better binding affinity to the enzyme 

Tyrosinase is an enzyme that catalyzes the oxidation of several molecules with phenolic groups. This enzyme plays a primary role in the browning of many fruits and vegetables. DOPA is one substrate of tyrosinase, undergoing the following reaction: OH OH + CH2 NH3 H -Coo DOPA tyrosinase + CO₂ -NH However, DOPA can be found as two different enantiomers, L-DOPA and D-DOPA. In a biochemistry lab, you measured the initial rate data given below for the L-DOPA and D-DOPA reactions with tyrosinase. Concentration of L-DOPA (mM) Initial Rate (µM/min) 10 20 12422 24.8 39.8 46.5 50.2 52.6 Concentration of D-DOPA(mM) Initial Rate (μM/min) 10 20 12422 7.05 12.2 20.8 37.9 41.7
Transcribed Image Text:Tyrosinase is an enzyme that catalyzes the oxidation of several molecules with phenolic groups. This enzyme plays a primary role in the browning of many fruits and vegetables. DOPA is one substrate of tyrosinase, undergoing the following reaction: OH OH + CH2 NH3 H -Coo DOPA tyrosinase + CO₂ -NH However, DOPA can be found as two different enantiomers, L-DOPA and D-DOPA. In a biochemistry lab, you measured the initial rate data given below for the L-DOPA and D-DOPA reactions with tyrosinase. Concentration of L-DOPA (mM) Initial Rate (µM/min) 10 20 12422 24.8 39.8 46.5 50.2 52.6 Concentration of D-DOPA(mM) Initial Rate (μM/min) 10 20 12422 7.05 12.2 20.8 37.9 41.7
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