Homework Help is Here – Start Your Trial Now!
Science
Biochemistry
From your Lineweaver-Burk plot, derive the values for the following table. Indicate the appropriate units. (b) By describing the potential changes in the kinetic parameters, identify and justify the type of inhibitor that was inhibiting the acid phosphatase activity.   Km Vmax Uninhibited     Inhibited

From your Lineweaver-Burk plot, derive the values for the following table. Indicate the appropriate units. (b) By describing the potential changes in the kinetic parameters, identify and justify the type of inhibitor that was inhibiting the acid phosphatase activity.   Km Vmax Uninhibited     Inhibited

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
See similar textbooks
icon
Related questions
Question

From your Lineweaver-Burk plot, derive the values for the following table. Indicate the appropriate units. (b) By describing the potential changes in the kinetic parameters, identify and justify the type of inhibitor that was inhibiting the acid phosphatase activity.

 

Km

Vmax

Uninhibited

 

 

Inhibited

 

 

 

 

 

1/ acid phosphate activity (min/nmol) -18 Lineweaver-Burk plot for acid phosphatase activity in the presence and in the absence of phosphate. -13 -8 -3 17 12 7 2 -3 -8 2 7 acid phosphate activity = 0.6323 x p-nitrophenylphosphate + 0.1017 presence of phosphate ion acid phosphate activity = 0.0318 x p-nitrophenylphosphate + 0.3312 absence of phosphate ion 1/ p-nitrophenylphosphate (L/mmol) I 12 17 22 27
Transcribed Image Text:1/ acid phosphate activity (min/nmol) -18 Lineweaver-Burk plot for acid phosphatase activity in the presence and in the absence of phosphate. -13 -8 -3 17 12 7 2 -3 -8 2 7 acid phosphate activity = 0.6323 x p-nitrophenylphosphate + 0.1017 presence of phosphate ion acid phosphate activity = 0.0318 x p-nitrophenylphosphate + 0.3312 absence of phosphate ion 1/ p-nitrophenylphosphate (L/mmol) I 12 17 22 27
Expert Solution
trending now

Trending now

This is a popular solution!

steps

Step by step

Solved in 3 steps

SEE SOLUTIONCheck out a sample Q&A here
Blurred answer
Similar questions
Recommended textbooks for you
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
Lehninger Principles of Biochemistry
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul…
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305961135
Author:
Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:
Cengage Learning
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305577206
Author:
Reginald H. Garrett, Charles M. Grisham
Publisher:
Cengage Learning
Fundamentals of General, Organic, and Biological …
Fundamentals of General, Organic, and Biological …
Biochemistry
ISBN:
9780134015187
Author:
John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson
Publisher:
PEARSON
SEE MORE TEXTBOOKS