The initial rate for an enzyme-catalyzed reaction has been determined at a number of substrate concentrations. Data are as follows: [S(u mol/L) v[(u mol/L) min-'] 5 22 10 39 20 65 50 102 100 120 200 135 (a) Estimate Vmax and K, from a direct graph of v versus [S]. Do you find difficulties in getting clear answers? (b) Now use a Lineweaver-Burk plot to analyze the same data. Does this work better?
The initial rate for an enzyme-catalyzed reaction has been determined at a number of substrate concentrations. Data are as follows: [S(u mol/L) v[(u mol/L) min-'] 5 22 10 39 20 65 50 102 100 120 200 135 (a) Estimate Vmax and K, from a direct graph of v versus [S]. Do you find difficulties in getting clear answers? (b) Now use a Lineweaver-Burk plot to analyze the same data. Does this work better?
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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![The initial rate for an enzyme-catalyzed reaction has been determined at a
number of substrate concentrations. Data are as follows:
[S(u mol/L)
v[(u mol/L) min-']
5
22
10
39
20
65
50
102
100
120
200
135
(a) Estimate Vmax and K, from a direct graph of v versus [S]. Do you find
difficulties in getting clear answers?
(b) Now use a Lineweaver-Burk plot to analyze the same data. Does this
work better?](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F3c36674e-3e5a-40fc-95de-e6a1a68d321e%2F2b5f9ca2-3e39-47a3-8984-a87b2e82ba27%2Fudeg60l_processed.png&w=3840&q=75)
Transcribed Image Text:The initial rate for an enzyme-catalyzed reaction has been determined at a
number of substrate concentrations. Data are as follows:
[S(u mol/L)
v[(u mol/L) min-']
5
22
10
39
20
65
50
102
100
120
200
135
(a) Estimate Vmax and K, from a direct graph of v versus [S]. Do you find
difficulties in getting clear answers?
(b) Now use a Lineweaver-Burk plot to analyze the same data. Does this
work better?
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