How do you use Lineweaver Burk plot to determine the Vmax and Km for both the uninhibited enzyme and the enzyme with “Inhibitor A”? Table 4.3Mean Rate (mm/minute) of ONPG hydrolysis catalyzed byß-Galactosidase at various ONPG concentrations with andwithout inhibitor. Each mean is the average of 36 replicates.ONPGConcentration(MM)0357.51015203040Mean Reaction Rate (mm/minute)SEMNoInhibitor (No Inhibitor)0.0000.0000.0070.0000.0120.0010.0150.0010.0200.0010.0270.0010.0330.0010.0450.0020.0460.001InhibitorA0.0000.0040.0060.0090.0120.0170.0210.0320.034SEM(Inhibitor A)0.0000.0000.0000.0010.0010.0010.0010.0010.001 1/Mean Reaction Rate (1/mm/min)300250200150100500-0.050.00◆no inhibitorInhibitor A0.050.100.150.201/[ONPG] (1/mM)y = 733.66x + 10.9190.25y = 390.8x + 10.770.300.35Figure 4.4: Lineweaver-Burk plot showing the effect of an inhibitor on hydrolysisof ONPG by b-galactosidase. Data points are the means of 36 replicates.0.40

The Lineweaver-Burk plot, also known as the double reciprocal plot, is a graphical representation of…

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Biochemistry

Lineweaver Burk plot to determine the Vmax and Km

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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