A researcher purifies a mutant version of the glucokinase enzyme. She combines the purified glucokinase with glucose and ATP to produce glucose 6–phosphate. She records the initial velocity (Vo) of glucose 6-phosphate production at various substrate concentrations ([S]). She determines a Michaelis–Menten constant (Km) of 6.2 mM for the mutant enzyme. umol [S] (mM) min 1.03 10 1.42 50 2.05 100 2.17 Calculate the maximum velocity (Vmax) of the mutant glucokinase to one decimal place using the Michaelis–Menten equation.

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